Purification and Characterization of Two Lectins from Callus of Helianthus tuberosus.

• NAKAGAWA Ryoji

• YASOKAWA Daisuke

• IKEDA Takayuki

• NAGASHIMA Koji

Bibliographic Information

Other Title

• Purification and Characterization of Two Lectins from Callus of<i>Helianthus tuberosus</i>

Abstract

Two lectins were purified from Helianthus tuberosus callus by maltose affinity chromatography and subsequent preparative electrophoresis. The lectins were designated HTAI and HTAII and their molecular masses were about 34 kDa by gel-filtration chromatography. A single band of 17 kDa and bands of 17 kDa and 18 kDa were detected after SDS-PAGE of HTA I and HTA II, respectively; indicating that HTAI is a homodimer while HTAII is a heterodimer. The amino acid compositions of the two lectins were very similar; they were rich in glycine residues, lacking detectable amounts of methionine, cysteine, and histidine. A hapten-inhibition assay showed that HTA I and HTA II had identical saccharide-binding specificity to the extent tested and belonged to the group of so-called mannose/glucose-binding lectins. They had high affinity forα-linked manno-oligosaccharides. Each HTA completely lost its hemagglutinatinig activity at pH 5.0, as a result of its dissociation to monomers, but it did not lose its ability to bind to oligosaccharides.

Journal

• Bioscience, Biotechnology, and Biochemistry

  Bioscience, Biotechnology, and Biochemistry 60 (2), 259-262, 1996

  Japan Society for Bioscience, Biotechnology, and Agrochemistry

Keywords

• Helianthus tuberosus
• compositae
• callus
• lectin
• characterization

Details

• CRID: 1390001206470639744
• NII Article ID: 110002677983
• NII Book ID: AA10824164
• DOI: 10.1271/bbb.60.259
• COI: 1:CAS:528:DyaK28XhtlKnur8%3D
• ISSN: 13476947
• ISSN: 09168451
• PubMed: 9063972
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.60.259
• Text Lang: en
• Data Source:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
• Abstract License Flag: Disallowed