Sensitive Analysis of Protein Phosphatase Inhibitors by the Firefly Bioluminescence System: Application to PP1<i>γ</i>

• SUGIYAMA Yukiteru

• FUJIMOTO Kazue

• OHTANI Ikuko I.

• TAKAI Akira

• ISOBE Minoru

書誌事項

タイトル別名

• Sensitive Analysis of Protein Phosphatase Inhibitors by the Firefly Bioluminescence System: Application to PP1.GAMMA..

抄録

Protein phosphatases are classified into types 1 (PP1) and 2 (PP2A, 2B, and 2C). We have already established a new analysis method for PP2A inhibitors by using the firefly bioluminescence system for detection. This method was successfully applied to determine the PP1_Y inhibitory activity of known inhibitors, i.e., calyculin A, microcystin-LR, and tautomycin, requiring less than 10 pmol of a sample.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 60 (8), 1260-1264, 1996

  公益社団法人 日本農芸化学会

キーワード

• protein phosphatase inhibitors
• bioluminescence
• PP1
• PP1<SUB>Y</SUB>
• tautomycin

詳細情報

• CRID: 1390282681453308928
• NII論文ID: 110002678241
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.60.1260
• ISSN: 13476947
• ISSN: 09168451
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.60.1260
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可