Purification and Characterization of Glycoprotein from the Skin Mucus of the Rainbow Trout,<i>Salmo gaivdneri</i>

• SUMI Toshihisa

• HAMA Yoichiro

• MARUYAMA Daisuke

• ASAKAWA Makio

• NAKAGAWA Hiroki

書誌事項

タイトル別名

• Purification and Characterization of Glycoprotein from the Skin Mucus of the Rainbow Trout, Salmo gairdneri.
• Purification and Characterization of Gl

抄録

Mucus glycoprotein (RGP) was purified and characterized from the skin mucus of rainbow trout, Salmo gairdneri. RGP was found to contain 30.1% NeuAc, 26.0% GalNAc, 5.0% Gal, and 26.0% amino acids. The protein moiety of RGP is very rich in Thr (32.4 mol%). Neither NeuGc nor KDN (2-keto-3-deoxy-D-glycero-D-galacto-nononic acid) was found in RGP. Alkaline borohydride treatment of RGP yielded a major disaccharide alditol, NeuAcx2→6GalNAc-ol and more than 4 minor oligosaccharide alditols including NeuAc→(GalNAcx1→)GalNAc-ol. It was evident that an average RGP molecule has approximately 500 NeuAc-containing oligosaccharide chains, which are attached to the Thr and Ser residues of the protein moiety and spaced at an average of 3 amino acids apart.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 61 (4), 675-683, 1997

  公益社団法人 日本農芸化学会

キーワード

• sialoglycoprotein
• mucus glycoprotein
• mucus
• rainbow trout
• mucin

詳細情報

• CRID: 1390282681451153152
• NII論文ID: 110002678553
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.61.675
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 4209503
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4209503
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.61.675
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可