Synthesis by a α-Glucosidase of Glycosyl-trehaloses with an Isomaltosyl Residue

• KURIMOTO Masashi

• NISHIMOTO Tomoyuki

• NAKADA Tetsuya

• CHAEN Hiroto

• FUKUDA Shigeharu

• TSUJISAKA Yoshio

書誌事項

タイトル別名

• Synthesis by an .ALPHA.-Glucosidase of Glycosyl-trehaloses with an Isomaltosyl Residue.
• Synthesis by a アルファ Glucosidase of Glyc
• Synthesis by an<i>α</i>-Glucosidase of Glycosyl-trehaloses with an Isomaltosyl Residue
• Synthesis by an α-glucosidase of glycosyl-trehaloses with an isomaltosyl residue

抄録

Glycosyl-trehaloses with an isomaltosyl residue were synthesized by α-glucosidase from Aspergillus niger by using maltotetraose as a glucosyl donor and trehalose as the acceptor. The one trisaccharide and two tetrasaccharides formed were isolated by successive column chromatography. The results of an enzymatic digestion, methylation analysis, and ¹³C-NMR studies indicated that these oligosaccharides were α-isomaltosyl α-glucoside, α-isomaltotriosyl α-glucoside and α-isomaltosyl α-isomaltoside. These oligosaccharides were not fermented to an acid by Streptococcus mutans, and they effectively inhibited water-insoluble glucan synthesis from sucrose by glucosyltransferase. In an in vitro utilization test with human intestinal bacteria, these oligosaccharides were predominantly utilized by Bifidobacteria.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 61 (4), 699-703, 1997

  公益社団法人 日本農芸化学会

キーワード

• trehalose
• isomaltosyl residue
• α-glucosidase

詳細情報

• CRID: 1390282681451147264
• NII論文ID: 110002678556
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.61.699
• COI: 1:CAS:528:DyaK2sXjtVyrur8%3D
• ISSN: 13476947
• ISSN: 09168451
• ISSN: http://id.crossref.org/issn/09168451
• NDL書誌ID: 4209506
• PubMed: 9145529
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4209506
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.61.699
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可