Characterization of 30-kDa Fragments Derived from β-Conglycinin Degradation Process during Germination and Seedling Growth of Soybean

• KAWAI Misako

• SUZUKI Shun'ichi

• ASANO Minao

• MIWA Tatsuya

• SHIBAI Hiroshiro

書誌事項

タイトル別名

• Characterization of 30-kDa Fragments Derived from .BETA.-Conglycinin Degradation Process during Germination and Seedling Growth of Soybean.
• Characterization of 30-kDa Fragments De
• Characterization of 30-kDa Fragments Derived from <i>β</i>-Conglycinin Degradation Process during Germination and Seedling Growth of Soybean

抄録

The degradation process of β-conglycinin, a vicilin-type glycosylated storage protein of soybean seeds, during germination and seedling growth was examined by concanavalin A blotting combined with polyacrylamide gel electrophoresis. We detected and analyied the structures of key intermediary fragments of 30 kDa derived from β-conglycinin degradation, they were proved to be single-domain type subunits of β-conglycinin. We show here a degradation process of β-conglycinin: β-conglycinin is subjected to limited proteolysis at exposed regions on the molecular surface, like domain junctions, generating 30-kDa singledomain fragments before non-specific proteolysis.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 61 (5), 794-799, 1997

  公益社団法人 日本農芸化学会

キーワード

• β-conglycinin
• soybean
• degradation
• proteolysis

詳細情報

• CRID: 1390001206474278144
• NII論文ID: 110002678581
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.61.794
• COI: 1:CAS:528:DyaK2sXjsVyisrk%3D
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 4220002
• PubMed: 9178555
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4220002
• Web Site: https://www.tandfonline.com/doi/pdf/10.1271/bbb.61.794
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可