Water Permeability of Plasma Membranes of Cultured Rice, Grape, and CH27 Cells Measured Dielectrically.

• ISHIKAWA Eisuke

• MIYAWAKI Osato

• NAKAMURA Kozo

書誌事項

タイトル別名

• Water Permeability of Plasma Membranes

抄録

The capacitance of suspensions of cultured rice cells (Oryza sativa L. ssp. japonica), grape cells (Vitis sp.), and CH27 cells originated from murine B-cell lymphoma was measured in the frequency range of 0.2 to 10 MHz. The relationship between the increase in capacitance caused by the presence of cells at 0.4 MHz, ΔC, and the cell density was linear. Measurement of capacitance was useful in measurement of transitional changes in cell volume under external osmotic stress when sucrose was added. From the course of volume changes with such stress, the water permeabilities of the plasma membrane, L_p, were measured to be 0.015, 0.020, and 0.090 pm/(s·Pa) at 25°C, for rice cells, grape cells, and CH27 cells, respectively. The smaller L_p for plant cells seemed to explain why preservation of plant cells by freezing is more difficult than for animal cells. From the temperature dependence of L_p, the apparent activation energies were calculated to be 12.0±2.9 and 13.0±5.2 kcal/mol for rice cells and CH27 cells, respectively.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 61 (11), 1826-1830, 1997

  公益社団法人 日本農芸化学会

キーワード

• freeze-induced dehydration
• freezing preservation of cells
• permittivity of cell suspension
• water permeability
• plasma membrane

詳細情報

• CRID: 1390001206472756352
• NII論文ID: 110002678825
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.61.1826
• COI: 1:CAS:528:DyaK2sXnslWrsb4%3D
• ISSN: 13476947
• ISSN: 09168451
• ISSN: http://id.crossref.org/issn/09168451
• NDL書誌ID: 4357337
• PubMed: 9404061
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4357337
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.61.1826
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可