Growth factors for a primary chick muscle cell culture from shochu distillery by-product

• MAHFUDZ Luthfi D.

• NAKASHIMA Kazuki

• OHTSUKA Akira

• HAYASHI Kunioki

書誌事項

タイトル別名

• Growth Factors for a Primary Chick Muscle Cell Culture from Shochu Distillery By-products.
• Growth Factors for a Primary Chick Musc

抄録

An unidentified growth factor (UGF) was separated from shochu distillery by-products (SDBP) and its effect on the growth of a primary chick muscle cell culture was investigated. Chick muscle cells were isolated from fertile eggs (13-day-old embryos) of commercial broilers. UGF was separated on Sephadex LH-20 with a solvent system of water-methanol-ethylene dichloride (10:90: 20, v/v), and the fraction eluted between 136 and 164 min was collected (fraction I). Fraction I was further purified by HPLC with an Inertsil ODS-2 column using a solvent system of methanol-butanol (80:20, v/v). Three fractions having retention times of 3.76, 4.57, and 5.12 min were collected and are referred to as fraction A, B, and C, respectively. In experiment 1, chick muscle cells were cultured in an m-199 medium containing 0.001, 0.01, or 0.1% of fraction I. In experiment 2, chick muscle cells were cultured with 0.01 or 0.005% of each fraction A, B, and C. Creatine kinase (CK) activity, protein and DNA contents were measured as indices of myotube growth, cell growth and cell proliferation, respectively. N^τ-mbthylhistidine (N^τ-MH) release from the muscle cell was also measured to observe the effect on proteolysis. In experiment 1, the protein content was significantly (p < 0.05) increased by fraction I, despite the low dose level. CK activity was significantly (p < 0.05) higher than the control when 0.001% of fraction I was added to the medium. However, increasing the level beyond 0.01% did not further increase the CK activity. The DNA content was not significantly changed. In experiment 2, the protein content, CK activity, and DNA content were significantly (p < 0.05) higher when fractions A and B were added to the medium. However, this was not the case when fraction C was added. N^τ-MH release was significantly (p < 0.05) higher when fraction A was added, but, was significantly (p < 0.05) lower when fraction B was added, while fraction C had no effect on N^τ-MH release. The present results show that SDBP contained two growth-promoting factors for a primary chick muscle cell culture, although their modes of action may be different.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 61 (11), 1844-1847, 1997

  公益社団法人 日本農芸化学会

キーワード

• muscle cell cultur
• unidentified growth factor
• distillery by-product

詳細情報

• CRID: 1390282681450065920
• NII論文ID: 110002678829
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.61.1844
• COI: 1:CAS:528:DyaK2sXnslWrsbo%3D
• ISSN: 13476947
• ISSN: 09168451
• ISSN: http://id.crossref.org/issn/09168451
• NDL書誌ID: 4357341
• PubMed: 9404063
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4357341
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.61.1844
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可