Purification and Characterization of an Isomaltotriose-producing<i>Endo</i>-dextranase from a<i>Fusarium</i>sp.
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- SHIMIZU Eishun
- Novo Nordisk Bioindustry Ltd.
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- UNNO Takehiro
- Research Institute, Nihon Shokuhin Kako Co., Ltd.
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- OHBA Manabu
- Department of Biology, Faculty of Education, Shizuoka University
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- OKADA Gentaro
- Department of Biology, Faculty of Education, Shizuoka University
書誌事項
- タイトル別名
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- Purification and Characterization of an Isomaltotriose-producing Endo-dextranase from a Fusarium sp.
- Purification and Characterization of an
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An isomaltotriose-producing endo-dextranase was simply purified from cell-free culture broth of a Fusarium sp. by ethanol fractionation and consecutive column chromatographies using DEAE-Toyopearl and Bio-Gel P-100. The purified enzyme was judged to be homogeneous on PAGE and SDS-PAGE as well as isoelectric focusing. The molecular mass of the enzyme was estimated to be about 69 kDa by SDS-PAGE. The enzyme is an acidic protein with a pI of 4.6. The optimum pH and temperature were pH 6.5 and 35°C, respectively. The enzyme was completely stable over the range of pH 4.5-11.8 at 4°C for 24 h and at temperatures below 45°C. Inactivation of the enzyme was found to be partial with 5 mM Cu2+, being about 70% inhibition and complete with 5 mM of Fe3+, Hg2+, Ag+ or NBS.<br> The enzyme split dextran in an endo-lytic action to produce a large amount of isomaltotriose and a slight amount of isomaltose and glucose. The anomeric configurations of the reaction products formed by the enzyme were α-form, indicating that the α-glycoside linkages in the substrate are retained. The final yield of isomaltotriose from dextran T-2000 was about 62%.<br>
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 62 (1), 117-122, 1998
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390282681453064320
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- NII論文ID
- 110002678926
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- NII書誌ID
- AA10824164
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- COI
- 1:CAS:528:DyaK1cXpsVCrug%3D%3D
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 4406233
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- PubMed
- 9501522
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可