Overproduction of 1,2-α-Mannosidase,a Glycochain Processing Enzyme,by Aspergillus oryzae

• YOSHIDA Takashi

  Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University

• NAKAJIMA Tasuku

  Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University

• ICHISHIMA Eiji

  Department of Applied Biological Chemistry, Faculty of Agriculture, Tohoku University Department of Bioengineering, Faculty of Engineering, Soka University

書誌事項

タイトル別名

• Overproduction of 1,2-.ALPHA.-Mannosidase, a Glycochain Processing Enzyme by Aspergillus oryzae.
• Overproduction of 1 2 アルファ Mannosidase
• Overproduction of 1,2-α-Mannosidase, a Glycochain Processing Enzyme, by<i>Aspergillus oryzae</i>

抄録

  A recombinant strain of Aspergillus oryzae has been constructed in which 1,2-α-mannosidase, an intracellular glycochain processing enzyme with specificity toward 1,2-α-mannosidic linkages, has been overexpressed. For the construction, the N-terminal signal-encoding sequence of the 1,2-α-mannosidase gene (msdC) from Penicillium citrinum was replaced with that of the aspergillopepsin I signal, and the fused gene was inserted between amyB promoter-terminator elements in the expression plasmid pTAPM1. A transformant of A. oryzae (the strain PM-1) secreted a great deal of heterogeneous 1,2-α-mannosidase into the culture media, which was purified by CM ion-exchange chromatography. Approximately 21 mg of the purified enzyme was obtained per liter of culture. N-terminal amino acid analysis indicated that the signal peptide was removed from the secreted enzyme. The Penicillium 1,2-α-mannosidase expressed in A. oryzae did not show any notable difference from the enzyme from P. citrinum in such properties as M_r, specific activity, CD spectra, or kinetic parameters. Man₇GlcNAc₂ accumulated temporarily during the degradation of Man₉GlcNAc₂ to Man₅GlcNAc₂ by fungal 1,2-α-mannosidase.<br>

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 62 (2), 309-315, 1998

  公益社団法人 日本農芸化学会

キーワード

• α-mannosidase
• expression
• fungi
• <i>Penicillium</i>
• <i>Aspergillus</i>

詳細情報

• CRID: 1390282681452610304
• NII論文ID: 110002678968
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.62.309
• COI: 1:CAS:528:DyaK1cXhslCmtrc%3D
• ISSN: 13476947
• ISSN: 09168451
• ISSN: http://id.crossref.org/issn/09168451
• NDL書誌ID: 4427626
• PubMed: 9532788
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4427626
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.62.309
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可