Purification and Characterization of Extracellular and Cell Wall Bound β-Glucosidases from Aspergillus kawachii

DOI Web Site Web Site PubMed 被引用文献33件 参考文献33件

書誌事項

タイトル別名
  • Purification and Characterization of Extracellular and Cell Wall Bound .BETA.-Glucosidases from Aspergillus kawachii.
  • Purification and Characterization of Extracellular and Cell Wall Bound ベーターGlucosidases from Aspergillus kawachii
  • Purification and Characterization of Extracellular and Cell Wall Bound β-Glucosidases from<i>Aspergillus kawachii</i>
  • Purification and characterization of extracellular and cell wall bound β-glucosidase from Aspergillus kawachii
  • Purification and characterization of extracellular and cell wall bond β-glucosidases from Aspergillus kawachii

この論文をさがす

抄録

  We isolated two extracellular β-glucosidases (EX-1: 145 kDa, EX-2: 130 kDa) and one cell wall bound β-glucosidase (CB-1: 120 kDa) from Aspergillus kawachii and characterized their physical and kinetic properties. From the results of N-terminal amino acid sequence, enzymatic parameters and deglycosylation of the three purified enzymes, we strongly suggest that these three enzymes were products of the same gene, modified by different degree of glycosylation. All three purified β-glucosidases adsorbed to the purified cell wall fraction of this strain. This association could dramatically improve the stability of purified enzymes. These three purified β-glucosidases were readily inactivated even in moderate conditions but became very stable upon the addition of the purified cell wall fraction. The all purified β-glucosidases were stable in the range of pH 2.0-9.0 and stable below 30°C with 2 mg/ml of the purified cell wall fraction.<br>

収録刊行物

被引用文献 (33)*注記

もっと見る

参考文献 (33)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ