Purification and Characterization of a Novel Cold-regulated Protein from an Ice-nucleating Bacterium,Pseudomonas fluorescens KUIN-1

• OBATA Hitoshi

  Department of Biotechnology, Faculty of Engineering, Kansai University Kansai University High Technology Research Center

• ISHIGAKI Hitoshi

  Department of Biotechnology, Faculty of Engineering, Kansai University

• KAWAHARA Hidehisa

  Department of Biotechnology, Faculty of Engineering, Kansai University Kansai University High Technology Research Center

• YAMADE Kazuhiro

  Department of Biotechnology, Faculty of Engineering, Kansai University

書誌事項

タイトル別名

• Purification and Characterization of a Novel Cold-regulated Protein from an Ice-nucleating Bacterium, <i>Pseudomonas fluorescens</i> KUIN-1

抄録

  The psychrotrophic ice-nucleating bacterium, Pseudomonas fluorescens KUIN-1 respond to a decrease in temperature with the induction of proteins that are classified as cold shock proteins (CSPs). We found the function of a 26-kDa protein of the CSPs in the strain KUIN-1. In strain KUIN-1, a cold shock from 18 to 4°C induced the synthesis of the 26-kDa protein. By analysis with SDS-PAGE, it was then demonstrated that the 26-kDa protein was produced by the cells after treatment at 4°C. The 26-kDa protein was purified to apparent homogeneity by (NH₄)₂SO₄ precipitation and some chromatographies (QA52, phenyl Superose, Superose 12, and Mono Q). The purified 26-kDa protein is composed of 6 subunits of 26.5-kDa with a molecular mass of approximately 159-kDa according to gel filtration and SDS-PAGE. The N-terminal sequence of the 26-kDa protein was Gln-Ala-Ala-Tyr-Tyr-Pro-Ala-His-His-His-Gln-Gln-Val-Gln-Gln-His-Trp-Gly-His-His-. Specifically, 26-kDa protein of the CSPs of strain KUIN-1 was very effective in protecting the cold-labile enzyme, lactate dehydrogenase against denaturation by freezing. The characteristics of 26-kDa protein are analogous to the cold-regulated protein of the plants.<br>

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 62 (11), 2091-2097, 1998

  公益社団法人 日本農芸化学会

キーワード

• ice-nucleating bacterium
• cold-regulated protein
• cold shock protein
• lactate dehydrogenase
• <i>Pseudomonas fluorescens</i> KUIN-1

詳細情報

• CRID: 1390001206474308224
• NII論文ID: 110002679085
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.62.2091
• COI: 1:CAS:528:DyaK1cXnvFymur4%3D
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 4667007
• PubMed: 9972230
• Web Site: http://id.ndl.go.jp/bib/4667007
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4667007
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.62.2091
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可