N-Carbamoyl-L-Cysteine as an Intermediate in the Bioconversion from D,L-2-Amino-Δ2-Thiazoline-4-Carboxylic Acid to L-Cysteine by Pseudomonas sp. ON-4a

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  • TAMURA Yoshiharu
    Nippon Rikagakuyakuhin Co., Ltd., Research Center
  • NISHINO Mizuka
    Department of Science of Bioresource, Faculty of Agriculture, Hirosaki University
  • OHMACHI Tetsuo
    Department of Science of Bioresource, Faculty of Agriculture, Hirosaki University
  • ASADA Yoshihiro
    Department of Science of Bioresource, Faculty of Agriculture, Hirosaki University

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タイトル別名
  • N-Carbamoyl-L-Cysteine as an Intermediate in the Bioconversion from D,L-2-Amino-.DELTA.2-Thiazoline-4-Carboxylic Acid to L-Cysteine by Pseudomonas sp. ON-4a.
  • N Carbamoyl L Cysteine as an Intermediate in the Bioconversion from D L 2 Amino デルタ 2 Thiazoline 4 Carboxylic Acid to L-Cysteine by Pseudomonas sp. ON-4a
  • <i>N</i>-Carbamoyl-<scp>L</scp>-Cysteine as an Intermediate in the Bioconversion from<scp>D</scp>,<scp>L</scp>-2-Amino-<i>Δ</i><sup>2</sup>-Thiazoline-4-Carboxylic Acid to<scp>L</scp>-Cysteine by<i>Pseudomonas</i>sp. ON-4a

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  We investigated the conversion of D,L-2-amino-Δ2-thiazoline-4-carboxylic acid (D,L-ATC) to L-cysteine with Pseudomonas sp. ON-4a, an ATC-assimilating bacterium. Cysteine and N-carbamoylcysteine (NCC), but not S-carbamoylcysteine (SCC), were produced from D,L-ATC by a cell-free extract from the strain. These products were isolated from the reaction mixture and then identified as the L-form. Similar results were obtained with P. putida AJ3865 and unidentified strain TG-3, an ATC-assimilating bacteria. It became clear that L-NCC is an intermediate in the conversion of D,L-ATC to L-cysteine in these Pseudomonas strains. Furthermore, it was suggested that these bacteria have L-ATC hydrolase and L-NCC amidohydrolase.<br>

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