Fluorescent Determination of Double-stranded DNA with an Anionic 1,1′-Azonaphthalene Derivative

• FUKUSHIMA Yasumasa

  Research & Development Center, Unitika Ltd.

• HAYASHI Mayumi

  Research & Development Center, Unitika Ltd.

• NAKAJIMA Hiroshi

  Research & Development Center, Unitika Ltd.

書誌事項

タイトル別名

• Fluorescent Determination of Double-stranded DNA with an Anionic 1,1'-Azonaphthalene Derivative.

抄録

  Palatine chrome black 6BN (PCB6BN) is virtually non-fluorescent in an aqueous solution or in the presence of single-stranded DNA (ssDNA), whereas the fluorescence intensity of PCB6BN was linearly enhanced up to 300 μM of double-stranded DNA (dsDNA) base pairs. PCB6BN could be a useful fluorescent probe for quantifying dsDNA even when ssDNA is present for both heterogeneous and homogeneous assays.<br>

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 62 (11), 2254-2256, 1998

  公益社団法人 日本農芸化学会

キーワード

• dsDNA determination
• anionic dye
• fluorescent probe

詳細情報

• CRID: 1390001206474403840
• NII論文ID: 110002679116
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.62.2254
• COI: 1:CAS:528:DyaK1cXnvFyls78%3D
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 4666800
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4666800
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.62.2254
• 本文言語コード: en
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