HPLC Method for Evaluation of the Free Radical-scavenging Activity of Foods by Using l,1-Diphenyl-2-picrylhydrazyl

• YAMAGUCHI Tomoko

  Graduate School of Human Culture, Nara Women’s University

• TAKAMURA Hitoshi

  Department of Food Science and Nutrition, Nara Women’s University

• MATOBA Teruyoshi

  Department of Food Science and Nutrition, Nara Women’s University

• TERAO Junji

  Department of Nutrition, School of Medicine, The University of Tokushima

書誌事項

タイトル別名

• HPLC Method for Evaluation of the Free Radical-scavenging Activity of Foods by Using 1,1-Diphenyl-2-picrylhydrazyl.
• HPLC Method for Evaluation of the Free

抄録

  An HPLC method for evaluation of the free radical-scavenging activity of foods by using 1,1-diphenyl-2-picrylhydrazyl (DPPH) is reported. The activity was evaluated by measuring the decrease of DPPH detected at 517 nm. By using this novel method, we determined the free radical-scavenging activity of several antioxidants: ascorbic acid, α-tocopherol, Trolox, and cysteine. The results gave good correlation between the radical-scavenging activity determined by HPLC and by conventional colorimetry. This methodology was applied to determine the free radical-scavenging activity of 8 beverages. The activity of coffee was the highest, followed by red wine, green tea, oolong tea, black tea, rosé wine, white wine, and orange juice. The results well agree with those of previous reports. This method is expected to be useful for a simple and rapid determination of free radical-scavenging activity in colored foods, because coloring substances in foods do not interfere with the measurement.<br>

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 62 (6), 1201-1204, 1998

  公益社団法人 日本農芸化学会

キーワード

• radical-scavenging activity
• 1,1-diphenyl-2-picrylhydrazyl
• antioxidant
• beverage

詳細情報

• CRID: 1390282681446297600
• NII論文ID: 110002679235
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.62.1201
• COI: 1:CAS:528:DyaK1cXksFCis74%3D
• ISSN: 13476947
• ISSN: 09168451
• ISSN: http://id.crossref.org/issn/09168451
• NDL書誌ID: 4514516
• PubMed: 9692204
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4514516
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.62.1201
• Web Site: https://search.jamas.or.jp/link/ui/1999085317
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可