Synthesis of Novel Heterobranched β-Cyclodextrins from α-D-Mannosyl-Maltotriose and β-Cyclodextrin by the Reverse Action of Pullulanase, and Isolation and Characterization of the Products

• KITAHATA Sumio

  Osaka Municipal Technical Research Institute

• TANIMOTO Toshiko

  School of Pharmaceutical Sciences, Mukogawa Women's University

• OKADA Yasuyo

  School of Pharmaceutical Sciences, Mukogawa Women's University

• IKUTA Akiko

  School of Pharmaceutical Sciences, Mukogawa Women's University

• TANAKA Keiko

  School of Pharmaceutical Sciences, Mukogawa Women's University

• MURAKAMI Hiromi

  Osaka Municipal Technical Research Institute

• NAKANO Hirofumi

  Osaka Municipal Technical Research Institute

• KOIZUMI Kyoko

  School of Pharmaceutical Sciences, Mukogawa Women's University

書誌事項

タイトル別名

• Synthesis of Novel Heterobranched .BETA.-Cyclodextrins from .ALPHA.-D-Mannosyl-Maltotriose and .BETA.-Cyclodextrin by the Reverse Action of Pullulanase, and Isolation and Characterization of the Products.
• Synthesis of Novel Heterobranched ベータ Cyclodextrins from アルファ D Mannosyl Maltotriose and ベータ Cyclodextrin by the Reverse Action of Pullulanase and Isolation and Characterization of the Products
• Synthesis of Novel Heterobranched β-Cyclodextrins from α-<scp>D</scp>-Mannosyl- Maltotriose and β-Cyclodextrin by the Reverse Action of Pullulanase, and Isolation and Characterization of the Products

抄録

  α-D-Mannosyl-maltotriose (Man-G3) were synthesized from methyl α-mannoside and maltotriose by the transfer action of α-mannosidase. (Man-G3)-βCD and (Man-G3)₂-βCD were produced in about 20% and 4% yield, respectively when Aerobacter aerogenes pullulanase (160 units per 1 g of Man-G3) was incubated with the mixture of 1.6 M Man-G3 and 0.16 M βCD at 50°C for 4 days. The reaction products, (Man-G3)-βCD were separated to three peaks by HPLC analysis on a YMC-PACK A-323-3 column and (Man-G3)₂-βCD were separated to several peaks by HPLC analysis on a Daisopak ODS column. The major product of (Man-G3)-βCDs was identified as 6-O-α-(6³-O-α-D-mannosyl-maltotriosyl)-βCD by FAB-MS and NMR spectroscopies. The structures of (Man-G3)₂-βCDs were analyzed by TOF-MS and NMR spectroscopies, and confirmed by comparison of elution profiles of their hydrolyzates by α-mannosidase and glucoamylase on a graphitized carbon column with those of the authentic di-glucosyl-βCDs. The structures of three main components of (Man-G3)₂-βCDs were identified as 6¹,6²-, 6¹,6³- and 6¹,6⁴-di-O-(6³-O-α-D-mannosyl-maltotriosyl)-βCD.<br>

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 64 (11), 2406-2411, 2000

  公益社団法人 日本農芸化学会

キーワード

• reverse action
• pullulanase
• heterobranched CD

詳細情報

• CRID: 1390001206473592960
• NII論文ID: 110002679832
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.64.2406
• COI: 1:CAS:528:DC%2BD3cXos1SrtLY%3D
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 5590451
• PubMed: 11193409
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I5590451
• Web Site: https://www.tandfonline.com/doi/pdf/10.1271/bbb.64.2406
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可