Cloning of a Full-length cDNA Encoding<i>ent</i>-Kaurene Synthase from<i>Gibberella fujikuroi</i>: Functional Analysis of a Bifunctional Diterpene Cyclase

• TOYOMASU Tomonobu

  Department of Bioresource Engineering, Yamagata University

• KAWAIDE Hiroshi

  Frontier Research Program, The Institute of Physical and Chemical Research (RIKEN)

• ISHIZAKI Atsuko

  Department of Bioresource Engineering, Yamagata University

• SHINODA Shoko

  Department of Bioresource Engineering, Yamagata University

• OTSUKA Minoru

  Department of Bioresource Engineering, Yamagata University

• MITSUHASHI Wataru

  Department of Bioresource Engineering, Yamagata University

• SASSA Takeshi

  Department of Bioresource Engineering, Yamagata University

書誌事項

タイトル別名

• Cloning of a Full-length cDNA Encoding ent-Kaurene Synthase from Gibberella fujikuroi: Functional Analysis of a Bifunctional Diterpene Cyclase.
• Cloning of a full-length cDNA encoding ent-kaurene synthase from Gibberella fujikuroi: functional analysis of a bifunctional deterpene cyclase

抄録

  We report here the nucleotide sequence of a full-length cDNA encoding ent-kaurene synthase that was isolated by a reverse-transcription polymerase chain reaction from Gibberella fujikuroi (Gcps/ks). This cDNA encodes 952 amino acid residues with a relative molecular mass of 107 kDa. The sequence similarity between Gcps/ks and ent-kaurene synthase of the gibberellin A₁-producing fungus, Phaeosphaeria sp. L487, is very high, suggesting that Gcps/ks is also a bifunctional diterpene cyclase. Its recombinant protein expressed in Escherichia coli converted geranylgeranyl diphosphate to copalyl diphosphate and ent-kaurene.<br>

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 64 (3), 660-664, 2000

  公益社団法人 日本農芸化学会

キーワード

• diterpene cyclase
• <i>ent</i>-kaurene synthase
• <i>Gibberella fujikuroi</i>
• gibberellin biosynthesis

詳細情報

• CRID: 1390001206474785024
• NII論文ID: 110002679973
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.64.660
• COI: 1:CAS:528:DC%2BD3cXit1GntLs%3D
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 5334508
• PubMed: 10803977
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I5334508
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.64.660
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可