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- NAKANO Hirofumi
- Osaka Municipal Technical Research Institute
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- SHIZUMA Motohiro
- Osaka Municipal Technical Research Institute
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- KISO Taro
- Osaka Municipal Technical Research Institute
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- KITAHATA Sumio
- Osaka Municipal Technical Research Institute
書誌事項
- タイトル別名
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- Galactosylation of Thiol Group by .BETA.-Galactosidase.
- Galactosylation of Thiol Group by ベータ Galactosidase
この論文をさがす
抄録
β-Galactosidase catalyzed β-galactosylation not only of a hydroxyl group but also of a thiol group in the condensation reaction of D-galactose and 2-mercaptoethanol. The thio-galactosylation product was confirmed as 2-hydroxyethyl S-β-D-galactoside on the bases of fast atom bombardment mass spectrometry, infrared spectroscopy, and nuclear magnetic resonance spectorometry. Aspergillus oryzae β-galactosidase hydrolyzed p-nitrophenyl S-β-D-galactoside most rapidly among several β-galactosidases and produced the thio-galactosylation product most efficiently. The Penicillim multicolor enzyme was as effective as the A. oryzae enzyme. However the enzymes from Escherichia coli, Saccharomyces fragilis, Kluyveromyces lactis, and Bacillus circulans galactosylated hydroxyl groups predominantly to produce O-galactoside. The thio-galactoside was synthesized most effectively at a 2-mercaptoethanol concentration of about 1.25 M. Galactose concentration at 0.8-2.8 M did not affect the synthetic yield of the thio-galactoside so greatly.<br>
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 64 (4), 735-740, 2000
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001206473686272
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- NII論文ID
- 110002679984
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- NII書誌ID
- AA10824164
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- COI
- 1:CAS:528:DC%2BD3cXjtVelsbo%3D
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- ISSN
- 13476947
- 09168451
- http://id.crossref.org/issn/09168451
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- NDL書誌ID
- 5379949
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- PubMed
- 10830485
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可