Synthesis of Novel Heterobranched β-Cyclodextrins from 42-ο-β-D-Galactosyl-Maltose and β-Cyclodextrin by the Reverse Action of Pullulanase, and Isolation and Characterization of the Products
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- KITAHATA Sumio
- Osaka Municipal Technical Research Institute
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- TANIMOTO Toshiko
- School of Pharmaceutical Sciences, Mukogawa Women’s University
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- IKUTA Akiko
- School of Pharmaceutical Sciences, Mukogawa Women’s University
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- TANAKA Keiko
- School of Pharmaceutical Sciences, Mukogawa Women’s University
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- FUJITA Koki
- Bio Research Corporation of Yokohama
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- HASHIMOTO Hitoshi
- Bio Research Corporation of Yokohama
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- MURAKAMI Hiromi
- Osaka Municipal Technical Research Institute
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- NAKANO Hirofumi
- Osaka Municipal Technical Research Institute
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- KOIZUMI Kyoko
- School of Pharmaceutical Sciences, Mukogawa Women’s University
書誌事項
- タイトル別名
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- Synthesis of Novel Heterobranched .BETA.-Cyclodextrins from 42-O-.BETA.-D-Galactosyl-Maltose and .BETA.-Cyclodextrin by the Reverse Action of Pullulanase, and Isolation and Characterization of the Products.
- Synthesis of Novel Heterobranched ベータ Cyclodextrins from 42 ベータ D Galactosyl Maltose and ベータ Cyclodextrin by the Reverse Action of Pullulanase and Isolation and Characterization of the Products
- Synthesis of Novel Heterobranched β-Cyclodextrins from 4<sup>2</sup>-<i>O</i>-β-<scp>D</scp>-Galactosyl- Maltose and β-Cyclodextrin by the Reverse Action of Pullulanase, and Isolation and Characterization of the Products
この論文をさがす
抄録
From the mixture of 42-O-β-D-galactosyl-maltose (Gal-G2) and β-cyclodextrin (βCD), novel heterobranched βCDs, (Gal-G2)-βCD and (Gal-G2)2-βCDs, were synthesized by the reverse action of debranching enzyme. The optimum conditions for the production of (Gal-G2)2-βCDs were examined. A mixture of (Gal-G2)2- βCDs was produced in about 4% yield when Aerobacter aerogenes pullulanase (64 units per 1 g of Gal-G2) was incubated with 1.6 M Gal-G2 and 0.16 M βCD at 50°C for 4 days. The reaction products, (Gal-G2)2-βCDs, were separated into three peaks by HPLC analysis on a Hypercarb S column. Their structures were analyzed by fast atom bombardment mass spectroscopy and NMR spectroscopies, and confirmed by comparison of their hydrolyzates by β-galactosidase with the authentic (G2)2-βCDs. The structures of (Gal-G2)-βCD and three components of (Gal-G2)2-βCDs were identified as 6-O-(Gal-G2)-βCD, 61,62-, 61,63- and 61,64-di-O-(Gal-G2)2-βCD, respectively.<br>
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 64 (6), 1223-1229, 2000
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390001206473049984
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- NII論文ID
- 110002680074
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- NII書誌ID
- AA10824164
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- COI
- 1:CAS:528:DC%2BD3cXks1Kqt78%3D
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 5446310
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- PubMed
- 10923794
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可