Phylogenetic Analysis of Methanogens in Sheep Rumen Ecosystem and Detection of<i>Methanomicrobium mobile</i>by Fluorescence<i>In Situ</i>Hybridization

• YANAGITA Kazuhiro

  National Institute of Bioscience and Human Technology, Agency of Industrial Science and Technology Department of Animal Sciences, Ibaraki University

• KAMAGATA Yoichi

  National Institute of Bioscience and Human Technology, Agency of Industrial Science and Technology

• KAWAHARASAKI Mamoru

  National Institute of Bioscience and Human Technology, Agency of Industrial Science and Technology

• SUZUKI Toshihiko

  National Institute of Bioscience and Human Technology, Agency of Industrial Science and Technology

• NAKAMURA Yutaka

  Department of Animal Sciences, Ibaraki University

• MINATO Hajime

  Department of Animal Sciences, Ibaraki University

書誌事項

タイトル別名

• Phylogenetic Analysis of Methanogens in Sheep Rumen Ecosystem and Detection of Methanomicrobium mobile by Fluorescence In Situ Hybridization.

抄録

  The population of methanogens in the sheep rumen microbial ecosystem was studied by using 16S rDNA cloning analysis, epifluorescence microscopy (which detects autofluorescence of a specific cofactor F₄₂₀ in methanogens) and the 16S rRNA-targeted in situ hybridization technique. The 16S rDNA clone libraries were constructed by PCR amplification with an Archaea-specific primer set and partial sequencing of the clonal 16S rDNAs was done. Phylogenetic analysis indicated that the clones were affiliated with Methanomicrobium mobile, Methanobrevibacter ruminantium and Methanobrevibacter smithii. Epifluorescence microscopy (F₄₂₀ autofluorescence) and in situ hybridization by using a newly designed M. mobile-specific 16S rRNA-targeted oligonucleotide probe found that methanogens accounted for approximately 3.6% of total ruminal microorganisms and approximately 54% of the total methanogens were M. mobile.<br>

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 64 (8), 1737-1742, 2000

  公益社団法人 日本農芸化学会

キーワード

• rumen
• methanogens
• <i>Methanomicrobium</i>
• <i>Methanobrevibacter</i>
• <i>in situ</i> hybridization

詳細情報

• CRID: 1390282681452328448
• NII論文ID: 110002680157
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.64.1737
• COI: 1:STN:280:DC%2BD3cvktlSktA%3D%3D
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 5512627
• PubMed: 10993166
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I5512627
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.64.1737
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可