Cloning and Analysis of Valerophenone Synthase Gene Expressed Specifically in Lupulin Gland of Hop(Humulus lupulus L.).

• OKADA Yukio

  Plant Bioengineering Research Laboratories, Sapporo Breweries Ltd.

• ITO Kazutoshi

  Plant Bioengineering Research Laboratories, Sapporo Breweries Ltd.

抄録

Resin and essential oil derived from hop (Humulus lupulus L.) cones are very important compounds for beer brewing, and they specifically accumulate in the lupulin gland of hop cones. In order to identify the genes responsible for the biosynthetic pathway of these compounds and use the identified genes for hop breeding using Marker Assisted Selection and transformation techniques, genes expressed specifically in the lupulin gland were cloned and sequenced. One of them was suggested to be similar to the chalcone synthase gene from the DNA sequence. The translation product of the gene had the activity of valerophenone synthase, which catalyzes a part of the synthesis reaction of α-acid and β-acid. Northern analysis showed that the valerophenone synthase gene seemed to be expressed specifically in the lupulin gland.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 65 (1), 150-155, 2001

  公益社団法人 日本農芸化学会

キーワード

• hop
• Humulus lupulus
• valerophenone synthase
• chalcone synthase
• α-acid

詳細情報

• CRID: 1390282681452215040
• NII論文ID: 110002680227
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.65.150
• COI: 1:CAS:528:DC%2BD3MXhtVGgt7c%3D
• ISSN: 13476947
• ISSN: 09168451
• ISSN: http://id.crossref.org/issn/13476947
• NDL書誌ID: 5659108
• PubMed: 11272819
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I5659108
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.65.150
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可