Sequence of the Clostridium thermocellum Mannanase Gene man26B and Characterizatin of the Translated Product
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- KUROKAWA Junji
- Faculty of Bioresources, Mie University
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- HEMJINDA Eiakalak
- Faculty of Bioresources, Mie University
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- ARAI Takamitsu
- Faculty of Bioresources, Mie University
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- KARITA Shuichi
- Center for Molecular Biology and Genetics, Mie University
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- KIMURA Tetsuya
- Faculty of Bioresources, Mie University
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- SAKKA Kazuo
- Faculty of Bioresources, Mie University
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- OHMIYA Kunio
- Faculty of Bioresources, Mie University
書誌事項
- タイトル別名
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- Sequence of the Clostridium thermocellum Mannanase Gene man26B and Characterization of the Translated Product.
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抄録
The man26B gene of Clostridium thermocellum strain F1 was found in pKS305, which had been selected as a recombinant plasmid conferring endoglucanase activity on Escherichia coli. The open reading frame of man26B consists of 1,773 nucleotides encoding a protein of 591 amino acids with a predicted molecular weight of 67,047. Man26B is a modular enzyme composed of an N-terminal signal peptide and three domains in the following order: a mannan-binding domain, a family 26 mannanase domain, and a dockerin domain responsible for cellulosome assembly. We found that this gene was a homologue of the man26A gene of C. thermocellum strain YS but that there were insertion or deletion mutations that caused a frame-shift mutation affecting a stretch of 26 amino acids in the catalytic domain. Man26B devoid of the dockerin domain was constructed and purified from a recombinant E. coli, and its enzyme properties were examined. Immunological analysis indicated that Man26B was a catalytic component of the C. thermocellum F1 cellulosome.
収録刊行物
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 65 (3), 548-554, 2001
公益社団法人 日本農芸化学会
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詳細情報 詳細情報について
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- CRID
- 1390282681450509184
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- NII論文ID
- 110002680302
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- NII書誌ID
- AA10824164
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- COI
- 1:CAS:528:DC%2BD3MXisFKqtbs%3D
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- ISSN
- 13476947
- 09168451
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- NDL書誌ID
- 5736572
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- PubMed
- 11330667
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- 本文言語コード
- en
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- データソース種別
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- 抄録ライセンスフラグ
- 使用不可