Green Marker for Colonies of Bacillus subtilis.

• ITAYA Mitsuhiro

  Mitsubishi Kasei Institute of Life Sciences

• SHAHEDUZZAMAN Syed M.

  Mitsubishi Kasei Institute of Life Sciences Present address: NCI, National Institutes of Health

• MATSUI Kuniko

  Mitsubishi Kasei Institute of Life Sciences

• OMORI Akira

  Mitsubishi Kasei Institute of Life Sciences

• TSUJI Takashi

  Mitsubishi Kasei Institute of Life Sciences

抄録

A Bacillus subtilis plasmid encoding a green fluorescence protein gene (gfp) was constructed. The fluorescence of B. subtilis colonies having this plasmid on agar plates was so high that they could be readily discerned visually under UV light. The fluorescence could be effectively expressed in three ways (i) through use of a strong bsr promoter (blasticidin S resistance gene), (ii) by efficient translation with the bsr translation system, and (iii) through increase in the copy number per cell. The high stability of the GFP plasmid was demonstrated by using more complicated growth conditions without any antibiotic for selection.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 65 (3), 579-583, 2001

  公益社団法人 日本農芸化学会

キーワード

• Bacillus subtilis
• green fluorescence protein
• fused protein
• multi-copy plasmid

詳細情報

• CRID: 1390282681450513152
• NII論文ID: 110002680306
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.65.579
• COI: 1:CAS:528:DC%2BD3MXisFKqtbc%3D
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 5737768
• PubMed: 11330671
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I5737768
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.65.579
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可