Expression Pattern of the CsPK3 Auxin-Responsive Protein Kinase Gene.

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  • CHONO Makiko
    Department of Applied Biological Chemistry, The University of Tokyo Bio-oriented Technology Research Advancement Institution (BRAIN)
  • SUZUKI Yoshihito
    Department of Applied Biological Chemistry, The University of Tokyo
  • NEMOTO Keisuke
    Asian Natural Environmental Science Center, The University of Tokyo
  • YAMANE Hisakazu
    Biotechnology Research Center, The University of Tokyo
  • MUROFUSHI Noboru
    Faculty of Bioresource Sciences, Akita Prefectural University
  • YAMAGUCHI Isomaro
    Department of Applied Biological Chemistry, The University of Tokyo

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We have previously cloned a cDNA of a putative serine/threonine protein kinase gene named CsPK3 from cucumber, the mRNA level of which was up-regulated by auxin and down-regulated by light irradiation. To examine the CsPK3 gene expression in detail, we cloned a genomic DNA of CsPK3 gene and made transgenic tobacco (Nicotiana tabacum L. cv. Petit Havana SR1) plants containing the fused CsPK3 promoter-β-glucuronidase gene. The β-glucuronidase expression was detected in the shoot apex, vascular tissues, and the outermost layer of cortex. The histological distribution of CsPK3 mRNA in cucumber seedlings was supported by in situ hybridization, where the positive signals were observed in similar tissues as those observed by β-glucuronidase staining. The responsiveness of the CsPK3 gene to auxin and light was also confirmed for β-glucuronidase activity. The pattern of β-glucuronidase staining changed during the development of the tobacco seedlings. The results of our experiment showed that CsPK3 was expressed in a wide variety of tissues and cells in which the developmental and growth controls by auxin are suggested.

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