Thermally Induced Changes of Lipoate Acetyltransferase Inner Core Isolated from the Bacillus stearothermophilus Pyruvate Dehydrogenase Complex

    • ASO Yoichi
    • Laboratory of Protein Chemistry and Engineering, Graduate School of Genetic Resources Technology, Kyushu University
    • NAKAJIMA Akihiro
    • Laboratory of Protein Chemistry and Engineering, Graduate School of Genetic Resources Technology, Kyushu University
    • MENO Kohji
    • Laboratory of Protein Chemistry and Engineering, Graduate School of Genetic Resources Technology, Kyushu University
    • ISHIGURO Masatsune
    • Laboratory of Protein Chemistry and Engineering, Graduate School of Genetic Resources Technology, Kyushu University

Abstract

Incubation at 70℃ converted the Bacillus stearothermophilus lipoate acetyltransferase inner core into an unidentified active molecular form, X, yielding an inactive aggregate. The core and X showed similar thermostabilities, but they were different in the recovery of enzyme activity after incubation with 1.2-2.0 M guanidine hydrochloride and its subsequent removal ; the core was hardly recovered, but X was well recovered.

Journal

Bioscience, biotechnology, and biochemistry   [List of Volumes]

Bioscience, biotechnology, and biochemistry 65(3), 698-701, 2001-03-23  [Table of Contents]

Japan Society for Bioscience, Biotechnology, and Agrochemistry

References:  12

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Codes

  • NII Article ID (NAID) :
    110002680329
  • NII NACSIS-CAT ID (NCID) :
    AA10824164
  • Text Lang :
    ENG
  • Article Type :
    NOT
  • ISSN :
    09168451
  • NDL Article ID :
    5738180
  • NDL Source Classification :
    ZR7(科学技術--農林水産--農産) // ZR2(科学技術--生物学--生化学) // ZP1(科学技術--化学・化学工業)
  • NDL Call No. :
    Z53-G223
  • DOI :
    10.1271/bbb.65.698
  • Databases :
    CJP  NDL  NII-ELS  J-STAGE 

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