Molecular Stability of Chicken and Rabbit Immunoglobulin G.

• SHIMIZU Makoto

• NAGASHIMA Hitoshi

• SANO Keisuke

• HASHIMOTO Kei

• OZEKI Makoto

• TSUDA Ken

• HATTA Hajime

抄録

Molecular stability of chicken egg yolk immunoglobulin G (IgY) and that of rabbit IgG were compared by measuring antibody activities and conformational changes. Stability of rabbit IgG to acid denaturation was much higher than that of IgY. Conformation of the IgY molecule was readily changed in acidic conditions, resulting in a rapid loss of antibody activity. Much less stable natures of IgY to heat-treatment and guanidine-HCl denaturation than rabbit IgG were also observed. Differences in the structure between the two immunoglobulins that might participate in their different stability were inferred from their amino acid sequence data. Importance of the intramoleular disulfide linkage in the rabbit light chain and some other structural differences were suggested.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 56 (2), 270-274, 1992

  公益社団法人 日本農芸化学会

キーワード

• Organic Chemistry
• Molecular Biology
• Applied Microbiology and Biotechnology
• General Medicine
• Biochemistry
• Analytical Chemistry
• Biotechnology

詳細情報

• CRID: 1390001206471551360
• NII論文ID: 110002680503
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.56.270
• COI: 1:CAS:528:DyaK38XhslWqs7k%3D
• ISSN: 13476947
• ISSN: 09168451
• ISSN: http://id.crossref.org/issn/09168451
• PubMed: 1368302
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.56.270
• Web Site: https://search.jamas.or.jp/link/ui/1994114023
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可