Partial Sequence of Acid Phosphatase-1¹Gene<i>(Aps</i>-1¹<i>)</i>Linked to Nematode Resistance Gene (<i>Mi</i>) of Tomato

• TANAKA Hiroshi

• HOSHI Jun'ichi

• NAKATA Kengo

• CHUNG Il. Kyung

• ITO Toru

• OHTA Akinori

• TAKAGI Masamichi

書誌事項

タイトル別名

• Partial Sequence of Acid Phosphatase-11 Gene(Aps-11)Linked to Nematode Resistance Gene(Mi)of Tomato.

抄録

A partial amino acid sequence of acid phosphatase-1¹ (apase-1¹), one of acid phosphatase isozymes of tomato, was identified. This information enabled us to synthesize degenerated primer pools of oligonucleotides for polymerase chain reactions (PCR) using cDNA for poly(A)⁺ RNA of tomato leaves as a template. As a result, a 135-bp, then a 467-hp PCR product were obtained. Nucleotide sequencing of these two PCR products gave a total of 522-bp sequence that was identified as a part of the Asp-1¹ gene judging from the amino acid sequence deduced from it. Using the 135-bp PCR product as a probe, we detected the restriction fragment length polymorphism (RFLP) in two different lines of tomato by genomic Southern blot analysis. We also did pulsed-field gel electrophoresis (PFGE) and Southern blot analysis to search for suitable fragments to clone into a YAC vector. As a result, a single band with a size that could be cloned into a YAC vector was detected when the genomic DNA was digested with some kinds of restriction enzymes.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 56 (4), 583-587, 1992

  公益社団法人 日本農芸化学会

キーワード

• Organic Chemistry
• Molecular Biology
• Applied Microbiology and Biotechnology
• General Medicine
• Biochemistry
• Analytical Chemistry
• Biotechnology

詳細情報

• CRID: 1390282681446058112
• NII論文ID: 110002680594
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.56.583
• ISSN: 13476947
• ISSN: 09168451
• PubMed: 1368210
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.56.583
• 本文言語コード: en
• データソース種別:
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  • Crossref
  • PubMed
  • CiNii Articles
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