Amino Acid Sequences of Trypsin Inhibitors from Oriental Pickling Melon (Cucumis melo L. var. Conomon MAKINO) Seeds

    • NISHINO Jiro
    • Department of Chemistry and Materials Technology, Faculty of Engineering and Design, Kyoto Institute of Technology
    • TAKANO Ryo
    • Department of Chemistry and Materials Technology, Faculty of Engineering and Design, Kyoto Institute of Technology
    • KAMEI-HAYASHI Kaeko
    • Department of Chemistry and Materials Technology, Faculty of Engineering and Design, Kyoto Institute of Technology

    • HARA Saburo
    • Department of Chemistry and Materials Technology, Faculty of Engineering and Design, Kyoto Institute of Technology

Abstract

Three inhibitors (CMCTI-I, II, and III) were isolated from oriental pickling melon (Cucumis melo L.var. Conomon MAKINO) seeds by acetone precipitation, gel filtration, and reversed phase chromatography. The amino acid sequences of these inhibitors were : [table] The reactive sites (P1 and P1' sites) of these inhibitors are presumed to be the Lys-Ile indicated by an arrow, comparing them with other squash family inhibitors. All three inhibitors can inhibit lysyl endopeptidase and trypsin at the enzyme-inhibitor ratio of 1 : 1.

Journal

Bioscience, biotechnology, and biochemistry   [List of Volumes]

Bioscience, biotechnology, and biochemistry 56(8), 1241-1246, 1992-08-23  [Table of Contents]

Japan Society for Bioscience, Biotechnology, and Agrochemistry

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Codes

  • NII Article ID (NAID) :
    110002680737
  • NII NACSIS-CAT ID (NCID) :
    AA10824164
  • Text Lang :
    ENG
  • ISSN :
    09168451
  • Databases :
    NII-ELS  Journal@rchive 

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