Biochemical Effects of High Hydrostatic Pressure on the Lysosome and Proteases Involved in It.

• OHMORI Takashi

• SHIGEHISA Tamotsu

• TAJI Siro

• HAYASHI Rikimaru

抄録

High pressure at 101.3 to 506.6MPa was applied to bovine liver and fractioned lysosomes to examine its effects on the lysosomes and the enzymes involved. Carboxypeptidase was completely inactivated at 405.3 MPa. The activities of about 60, 70, 30, and 5O% of acid phosphatase and cathepsins B, D, and H, respectively, remained after pressurization at 506.6 MPa. Pressurization of the liver at 506.6 MPa or lower caused changes in the subcellular distribution of lysosomal enzymes. Acid phosphatase and cathepsins B and H were released from fractioned lysosome by treatment higher than 202.7 MPa and cathepsin D at 101.3MPa. These findings indicate that high pressure at 101.3 to 202.7MPa induces destruction of the lysosome membranes and leakage of the lysosomal enzymes.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 56 (8), 1285-1288, 1992

  公益社団法人 日本農芸化学会

キーワード

• Organic Chemistry
• Molecular Biology
• Applied Microbiology and Biotechnology
• General Medicine
• Biochemistry
• Analytical Chemistry
• Biotechnology

詳細情報

• CRID: 1390001206471912704
• NII論文ID: 110002680746
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.56.1285
• COI: 1:CAS:528:DyaK3sXnsF2iuw%3D%3D
• ISSN: 13476947
• ISSN: 09168451
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.56.1285
• 本文言語コード: en
• データソース種別:
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  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可