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Methanosarcina barkeri strain Fusaro was grown on a mixed substrate medium of methanol and acetate. When 50mM of acetate was added to the methanol basal medium(250mM), the rates of methane production, methanol consumption, cell growth and corrinoid production were stimulated 3.2,2.7,3.5,and 2.4 times, respectively compared with those in methanol alone. Addition of acetate also has a significant influence on corrinoid distribution decreasing the intracellular corrinoid content from 6.8 to 3.0mg/g dry cell and increasing the extracellular corrinoid concentration from 4.0 to 5.4mg/l. The carbon balance analysis for methanogenesis and cellular growth with or without acetate addition revealed that about 50% of the utilized acetate carbon might be incorporated in the cellular materials and the remaining might by oxidized to generate the electrons which stimulate the methanol reduction to methane, accelerating the metabolic activities of the methanogenesis from methanol consequently enhancing the rates of methane and corrinoid production, and cell growth.