Geminate Particle Morphology of Sweet Potato Leaf Curl Virus in Partially Purified Preparation and Its Serological Relationship to Two Begomoviruses by Western Blotting :

• ONUKI,Masatoshi

  Kyushu National Agricultural Experiment Station

• HONDA,Yohachiro

  National Agriculture Research Center

• HANADA,Kaoru

  Kyushu National Agricultural Experiment Station

抄録

Sweet potato leaf curl virus(SPLCV)is a possible member of the genus Begomovirus;however, the presence of typical geminate particles in sap has not been confirmed. We attempted to observe SPLCV virions by partially purifying the virus using an enzyme-assisted procedure. The observed virions in the partially purified preparations were typical geminate particles with a size of ca.18×30nm. This virus preparation was subjected to western blot analysis using antisera against Bean golden mosaic virus(BGMV)and Mungbean yellow mosaic virus(MYMV). SPLCV reacted with both antisera. Specific bands appeared to be slightly larger than the 30-kDa marker protein and were considered to be SPLCV coat protein. This western blot analysis revealed for the first time a serelogical relationship between SPLCV and the two well-characterized begomoviruses.

収録刊行物

• Journal of general plant pathology : JGPP

  Journal of general plant pathology : JGPP 66 (2), 182-184, 2000-05-25

  Phytopathological Society of Japan

キーワード

• Sweet potato leaf curl virus
• begomovirus
• partial purification
• western blotting

詳細情報

• CRID: 1542261570221554688
• NII論文ID: 110002682893
• NII書誌ID: AA11449093
• Web Site: http://dl.ndl.go.jp/info:ndljp/pid/10774642
• 本文言語コード: en
• データソース種別:
  • NDL-Digital
  • CiNii Articles