Molecular Phylogenetic Analysis of Ribosomal DNA Internal Transcribed Spacer Regions and Comparison of Fertility in Phomopsis Isolates from Fruit Trees :

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Sequences of the ribosomal DNA(rDNA)internal transcribed spacer(ITS)regions were examined to infer a molecular phylogeny of small-spored Phomopsis isolates, designated W-type(mainly white colony, weakly virulent, bearing both alpha and beta conidia at 25℃ on PDA)and G-type(mainly gray colony, highly virulent, bearing only alpha conidia at 25℃ on PDA), and P.amygdali from fruit trees. Phomopsis G-type and P.amygdali were a monophyletic group distinct from the W-type. The W-type isolates were divided into two monophyletic groups. Diaporthe citri, D.tanakae, P.asparagi, P.viticola, P.vitimegaspora and D.nomurai, which are morphologically distinguishable from W- and G-types, differed from the W- and G-types in molecular phylogenetic analyses. PCR-RFLP analysis of rDNA ITS regions was useful to distinguish each of the Phomopsis species and groups using three restriction enzymes. In mating tests, W-type isolates from fruit trees were heterothallic and inter-fertile even between isolates belonging to the different monophyletic groups. Isolates of the G-type and P.amygdali collected in Japan were cross-fertile. Some isolates from Lunaria annua, Ulmus glabra and Juglans regia belonged to one of the two monophyletic groups of the W-type and were cross-fertile with W-type isolates from Rosaceous fruit trees.

Sequences of the ribosomal DNA(rDNA)internal transcribed spacer(ITS)regions were examined to infer a molecular phylogeny of small-spored Phomopsis isolates, designated W-type(mainly white colony, weakly virulent, bearing both alpha and beta conidia at 25℃ on PDA)and G-type(mainly gray colony, highly virulent, bearing only alpha conidia at 25℃ on PDA), and P.amygdali from fruit trees. Phomopsis G-type and P.amygdali were a monophyletic group distinct from the W-type. The W-type isolates were divided into two monophyletic groups. Diaporthe citri, D.tanakae, P.asparagi, P.viticola, P.vitimegaspora and D.nomurai, which are morphologically distinguishable from W- and G-types, differed from the W- and G-types in molecular phylogenetic analyses. PCR-RFLP analysis of rDNA ITS regions was useful to distinguish each of the Phomopsis species and groups using three restriction enzymes. In mating tests, W-type isolates from fruit trees were heterothallic and inter-fertile even between isolates belonging to the different monophyletic groups. Isolates of the G-type and P.amygdali collected in Japan were cross-fertile. Some isolates from Lunaria annua, Ulmus glabra and Juglans regia belonged to one of the two monophyletic groups of the W-type and were cross-fertile with W-type isolates from Rosaceous fruit trees.

収録刊行物

  • Journal of general plant pathology : JGPP

    Journal of general plant pathology : JGPP 66(3), 191-201, 2000

    Phytopathological Society of Japan

参考文献:  44件中 1-44件 を表示

被引用文献:  5件中 1-5件 を表示

各種コード

  • NII論文ID(NAID)
    110002682895
  • NII書誌ID(NCID)
    AA11449093
  • 本文言語コード
    ENG
  • 資料種別
    ART
  • ISSN
    13452630
  • データ提供元
    CJP書誌  CJP引用  NII-ELS  IR  NDL-Digital 
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