Disease Resistance of Tissue-cultured Seedlings of Rhododendron after Treatment with Streptomyces sp. R-5 :
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- SHIMIZU,Masafumi
- Laboratory of Ecological Circulation, Mie University
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- FUJITA,Norikazu
- Laboratory of Ecological Circulation, Mie University
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- NAKAGAWA,Yoshiko
- Akatsuka Orchid Co.Ltd.
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- NISHIMURA,Tomio
- Akatsuka Orchid Co.Ltd.
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- FURUMAI,Tamotsu
- College of Technology, Toyama Prefectural University
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- IGARASHI,Yasuhiro
- College of Technology, Toyama Prefectural University
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- ONAKA,Hiroyasu
- College of Technology, Toyama Prefectural University
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- YOSHIDA,Ryuji
- Biotechnology Research Center, Toyama Prefectural University
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- KUNOH,Hitoshi
- Labiratory of Ecological Circulation, Mie University
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抄録
An endophytic actinomycete, Streptomyces sp. R-5, which had been isolated from a field-grown rhododendron plant, was used to protect rhododendron seedlings in tissue culture from Pestalotia disease caused by Pestalotiopsis sydowiana. R-5 had intense antagonistic activity against P. sydowiana without adversely affecting the seedlings in glass flasks. A suspension of R-5 was spread on the surface of the multiplication medium in glass flasks in which seedlings were growing. Ten days later, the 4th upper leaf of seedlings was inoculated with P. sydowiana and incubated for 14 days. In controls untreated with R-5, substrate mycelia of P. sydowiana grew on all leaves and stems above and below the 4th leaf within 2-3 days of inoculation. Such growth resulted in the wilting death of 54% of seedlings by 14 days. In contrast, only the inoculated leaves turned brown in ca. 90% of seedlings growing on medium treated with R-5. None of these seedlings died. Thus, treatment of the medium surface with R-5 efficiently protects the seedlings from infection by P. sydowiana. Scanning electron microscopy revealed that substrate mycelia of R-5 gnew on and beneath the cuticle of leaves of the treated scedlings. Fluorescent microscopy showed that R-5 was also inside the leaves.
収録刊行物
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- Journal of general plant pathology : JGPP
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Journal of general plant pathology : JGPP 67 (4), 325-332, 2001-11-25
Phytopathological Society of Japan
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詳細情報 詳細情報について
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- CRID
- 1542261570221572992
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- NII論文ID
- 110002682990
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- NII書誌ID
- AA11449093
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- 本文言語コード
- en
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- データソース種別
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- NDL-Digital
- CiNii Articles