cDNA Cloning and Genetic Analysis of a Novel Decolorizing Enzyme, Peroxidase Gene dyp from Geotrichum candidum Dec 1

A novel decolorizing peroxidase gene(dyp)was cloned from a cDNA library of a newly isolated strain of fungus Geotrichum candidum Dec 1. The open reading frame of 1494 nucleotides which corresponds to dyp predicts a primarytranslation product of 498 amino acids, M_r 53, 306. The deduced amino acid sequence of DyP does not contain the typical conserved motif which is characteristic of heme-containing peroxidases in the plant peroxidase superfamily. Comparison of the deduced amino acid sequence of DyP with that of a peroxidase from Polyporaceae sp.suggests that these proteins share highly homologous regions.