Purification and Characterization of a DNA-dependent RNA Polymerase from<i>Pseudomonas putida</i>

• FUJITA Masaya

• AMEMURA Akinori

書誌事項

タイトル別名

• Purification and Characterization of a DNA-dependent RNA Polymerase from Pseudomonas putida.

抄録

DNA-dependent RNA polymerase (EC 2.7.7.6) was purified from Pseudomonas putida. The enzyme had the typical composition of β', β, α, and σ subunits of eubacterial RNA polymerases. The molecular masses of the subunits were 156, 000 Da, 151, 000 Da, 87, 000 Da, and 42, 000 Da, respectively, as measured by polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate. The NH₂-terminal amino acid residues of the α subunit had a marked homology with those of the α subunit of Escherichia coli RNA polymerase. The enzyme activity was dependent on ribonucleoside triphosphates, Mg²⁺, and a DNA template, and was inhibited in vitro by rifampicin. The enzyme activity was maximal in the presence of 10 mM MgCl₂. In an in vitro transcription assay using the tac promoter-controlled DNA as a template, the RNA polymerase of P. putida initiated transcription at the same site as that of E. coli.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 56 (11), 1797-1800, 1992

  公益社団法人 日本農芸化学会

キーワード

• Organic Chemistry
• Molecular Biology
• Applied Microbiology and Biotechnology
• General Medicine
• Biochemistry
• Analytical Chemistry
• Biotechnology

詳細情報

• CRID: 1390001206472173952
• NII論文ID: 110002691669
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.56.1797
• ISSN: 13476947
• ISSN: 09168451
• PubMed: 1369075
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.56.1797
• 本文言語コード: en
• データソース種別:
  • JaLC
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可