Purification and Characterization of a NAD⁺-Dependent Formate Dehydrogenase Produced by<i>Paracoccus</i>sp.

• IIDA Mitsugi

• KITAMURA-KIMURA Keiko

• MAEDA Hidekatsu

• MINEKI Shigeru

書誌事項

タイトル別名

• Purification and Characterization of a NAD+-Dependent Formate Dehydrogenase Produced by Paracoccus sp..

抄録

A facultative formate-utilizing bacterium, Paracoccus sp. strain 12-A, with a high specific activity (11.6 units/mg) of NAD⁺-dependent formate dehydrogenase, was isolated from sewage. From a crude extract of the cells, the enzyme was purified to homogeneity by ammonium sulfate fractionation, DEAE-cellulose column chromatography and hydroxyapatite column chromatography. The enzyme protein amounted to 15% of intracellular soluble proteins in strain 12-A. The enzyme had a molecular weight of approximately 100, 000 by gel filtration with Sephadex G-150 and 49, 000 by SDS-polyacrylamide gel electrophoresis, and an isoelectric point of 5.4. The pH and temperature optima ware 6.5-7.5 abd 50-55°C, respectively. The enzyme was stable at pH 6.0-10.0 and up to 50°C. The apparent K_m values for formate and NAD⁺ were calculated to be 5.0 mM and 0.036 mM, respectively.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 56 (12), 1966-1970, 1992

  公益社団法人 日本農芸化学会

キーワード

• Organic Chemistry
• Molecular Biology
• Applied Microbiology and Biotechnology
• General Medicine
• Biochemistry
• Analytical Chemistry
• Biotechnology

詳細情報

• CRID: 1390282681446854912
• NII論文ID: 110002691714
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.56.1966
• COI: 1:CAS:528:DyaK3sXhs1ersrc%3D
• ISSN: 13476947
• ISSN: 09168451
• ISSN: http://id.crossref.org/issn/09168451
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.56.1966
• 本文言語コード: en
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