Isolation,Characterization,and Sugar Chain Structure of EndoPG 1a,1b,and 1c from Stereum purpureum

• HASUI Yuji

  Department of Bioresources Science, Faculty of Agriculture, Hirosaki University

• FUKUI Yohko

  Department of Bioresources Science, Faculty of Agriculture, Hirosaki University

• KIKUCHI Junko

  Department of Bioresources Science, Faculty of Agriculture, Hirosaki University

• KATO Nozomu

  Department of Bioresources Science, Faculty of Agriculture, Hirosaki University

• MIYAIRI Kazuo

  Department of Bioresources Science, Faculty of Agriculture, Hirosaki University

• OKUNO Toshikatsu

  Department of Bioresources Science, Faculty of Agriculture, Hirosaki University

書誌事項

タイトル別名

• Isolation, Characterization, and Sugar Chain Structure of EndoPG Ia, Ib, and Ic from Stereum purpureum.
• IsolationCharacterizationand Sugar Chai
• Isolation, Characterization, and Sugar Chain Structure of EndoPG Ia, Ib, and Ic from<i>Stereum purpureum</i>

抄録

  Three endopolygalacturonases (endoPG Ia, Ib, and Ic) were isolated from the culture filtrate of Stereum purpureum, the causative fungus of apple silver-leaf disease. Their properties, including specific activities, optimum pHs, thermal stabilities, and kinetic parameters (K_m and V_max) were compared. Their properties were very similar to one another except for the substrate specificity and relative molecular mass. The sugar chains of endoPG Is were released by hydrazinolysis, and one major sugar chain common to endoPG Is was isolated. The pyridylamino sugar was characterized by a two-dimensional mapping method using HPLC, and identified as a high mannose type N-linked sugar chain, Manα1-6(Manα1-3)Manα1-6(Manα1-3) Manβ1-4GlcNAcβ1-4 GlcNAc (designated as M5.1). Observation of the course of Western blot analysis for the proteins from the culture filtrate with endoPG I antibodies showed that the fungus secreted three endoPG Is into the culture broth during the growing period.<br>

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 62 (5), 852-857, 1998

  公益社団法人 日本農芸化学会

キーワード

• endopolygalacturonase Ia
• endopolygalacturonase Ib
• endopolygalacturonase Ic
• N-linked sugar chain
• <i>Stereum purpureum</i>

詳細情報

• CRID: 1390282681451098240
• NII論文ID: 110002691905
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.62.852
• COI: 1:CAS:528:DyaK1cXktV2itL0%3D
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 4514261
• PubMed: 9648215
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4514261
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.62.852
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可