γ-Glutamyl Transfer Reactions by Glutaminase from Pseudomonas nitroreducens IFO 12694 and Their Application for the Syntheses of Theanine and γ-Glutamylmethylamide

• TACHIKI Takashi

  Department of Bioscience and Biotechnology, Faculty of Science and Engineering, Ritsumeikan University

• YAMADA Takeshi

  Department of Bioscience and Biotechnology, Faculty of Science and Engineering, Ritsumeikan University

• MIZUNO Katsushige

  Department of Bioscience and Biotechnology, Faculty of Science and Engineering, Ritsumeikan University

• UEDA Masashi

  Department of Bioscience and Biotechnology, Faculty of Science and Engineering, Ritsumeikan University

• SHIODE Ju-ichi

  Department of Bioscience and Biotechnology, Faculty of Science and Engineering, Ritsumeikan University

• FUKAMI Hiroshi

  Department of Bioscience and Biotechnology, Faculty of Science and Engineering, Ritsumeikan University

書誌事項

タイトル別名

• .GAMMA.-Glutamyl Transfer Reactions by Glutaminase from Pseudomonas nitroreducens IFO 12694 and Their Application for the Syntheses of Theanine and .GAMMA.-Glutamylmethylamide.
• ガンマ Glutamyl Transfer Reactions by Glut
• γ-Glutamyl Transfer Reactions by Glutaminase from<i>Pseudomonas nitroreducens</i>IFO 12694 and Their Application for the Syntheses of Theanine and γ-Glutamylmethylamide

抄録

  In a mixture containing γ-glutamyl donor (donor) and γ-glutamyl acceptor (acceptor), the glutaminase of Pseudomonas nitroreducens IFO 12694 simultaneously catalyzed a γ-glutamyl transfer reaction and hydrolysis of the donor. The variation of the activities responding to the concentration of glutathione and glycylglycine indicated that the enzyme might be classified in a group of glutaminases that shows hydrolysis prior to transfer reaction. On the other hand, the results with glutamine and ethylamine or methylamine indicated that the enzyme was active in the transfer reaction with suppressed hydrolysis of glutamine, and suggested the possibility of using the reaction for producing γ-glutamylethylamide (theanine) or γ-glutamylmethylamide (γ-GMA). In fact, in a mixture containing high concentrations of substrates (0.7 M glutamine, 1.5 M ethylamine or methylamine) and 0.5 unit/ml glutaminase (borate buffer pH 11), 270 mM (47 g/L) theanine or 250 mM (38 g/L) γ-GMA was formed in 7 h of incubation at 30°C.<br>

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 62 (7), 1279-1283, 1998

  公益社団法人 日本農芸化学会

キーワード

• theanine
• γ-glutamylmethylamide
• glutaminase
• γ-glutamyl transfer reaction
• <i>Pseudomonas nitroreducens</i>

詳細情報

• CRID: 1390001206475844096
• NII論文ID: 110002691944
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.62.1279
• COI: 1:CAS:528:DyaK1cXltFGht74%3D
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 4538406
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4538406
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.62.1279
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可