Hydroxyl Radical-scavenging Effects of Spices and Scavengers from Brown Mustard(Brassica nigra)

• Kim Jae Whune

• Minamikawa Takao

書誌事項

タイトル別名

• Hydroxyl Radical-scavenging Effects of Spices and Scavengers from Brown Mustard <i>(Brassica nigra)</i>
• Hydroxyl Radical-scavenging Effects of

抄録

When suspension cells of French bean (Phaseolus vulgaris L. cv. Goldstar) were cultured in Murashige and Skoog (MS) liquid medium containing 10^-6 M 1-naphthaleneacetic acid (NAA) and 10^-6 M 6-benzylaminopurine (BAP) (control medium), endopeptidase activity was expressed throughout the cell growth. In the medium containing 10^-5 M gibberellic acid (GA₃), the endopeptidase activity increased notably during the cell culture, but in the medium containing amino acids the activity decreased. Protein immunoblotting with an antiserum raised against a French bean cysteine endopeptidase (EP-C1) showed that a 34-kDa polypeptide corresponding to EP-C1 in molecular mass occurred in extracts from cultured cells. Five forms of endopeptidase in extracts of cells cultured in the control medium and the four forms in the medium containing GA₃ were detected by activity staining after non-denaturing polyacrylamide gel electrophoresis. Experiments with various protease inhibitors indicated that a serine endopeptidase was expressed at high levels in cultured cells in the control medium and the activity of a cysteine endopeptidase was increased by GA₃.

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 61 (1), 118-123, 1997

  公益社団法人 日本農芸化学会

キーワード

• endopeptidase
• gibberellic acid
• Phaseolus vulgaris
• protease
• suspension cells

詳細情報

• CRID: 1390282681448333952
• NII論文ID: 110002693212
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.61.118
• COI: 1:CAS:528:DyaK2sXhtVSjsbk%3D
• ISSN: 13476947
• ISSN: 09168451
• ISSN: http://id.crossref.org/issn/09168451
• NDL書誌ID: 4153731
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I4153731
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.61.118
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可