Transepithelial Transport of the Bioactive Tripeptide, Val-Pro-Pro, in Human Intestinal Caco-2 Cell Monolayers.

• SATAKE Makoto

  <i>Department of Applied Biological Chemistry, The University of Tokyo</i>

• ENJOH Masashi

  <i>Department of Applied Biological Chemistry, The University of Tokyo</i>

• NAKAMURA Yasunori

  <i>Research and Development Center, The Calpis Co. Ltd.</i>

• TAKANO Toshiaki

  <i>Research and Development Center, The Calpis Co. Ltd.</i>

• KAWAMURA Yukio

  <i>Department of Applied Life Science, Kinki University</i>

• ARAI Soichi

  <i>Department of Applied Biological Chemistry, The University of Tokyo</i>

• SHIMIZU Makoto

  <i>Department of Applied Biological Chemistry, The University of Tokyo</i>

抄録

  Some of the food-derived tripeptides with angiotensin converting enzyme (ACE)-inhibitory activity have been reported to be hypotensive after being orally administered. The mechanism for the intestinal transport of these tripeptides was studied by using monolayer- cultured human intestinal Caco-2 cells which express many enterocyte-like functions including the peptide transporter(PepT1)-mediated transport system. Val-Pro-Pro, an ACE-inhibitory peptide from fermented milk, was used as a model tripeptide. A significant amount of intact Val-Pro-Pro was transported across the Caco-2 cell monolayer. This transport was hardly inhibited by a competitive substrate for PepT1. Since no intact Val-Pro-Pro was detected in the cells, Val-Pro-Pro apically taken by Caco-2 cells via PepT1 was likely to have been quickly hydrolyzed by intracellular peptidases, producing free Val and Pro. These findings suggest that PepT1-mediated transport was not involved in the transepithelial transport of intact Val-Pro-Pro. Paracellular diffusion is suggested to have been the main mechanism for the transport of intact Val-Pro-Pro across the Caco-2 cell monolayer.<br>

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 66 (2), 378-384, 2002

  公益社団法人 日本農芸化学会

キーワード

• tripeptide
• bioactive peptide
• peptide transporter
• paracellular pathway
• Caco-2 cell

詳細情報

• CRID: 1390001206475467008
• NII論文ID: 110002693657
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.66.378
• COI: 1:CAS:528:DC%2BD38XitVWls7s%3D
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 6088038
• PubMed: 11999412
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6088038
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.66.378
• Web Site: https://search.jamas.or.jp/link/ui/2003000006
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
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