Identification of a Novel Anti-ice-nucleating Polysaccharide from Bacillus thuringiensis YY529.
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- YAMASHITA Yasuhiro
- <i>Department of Biotechnology, Faculty of Engineering, Kansai University</i>
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- KAWAHARA Hidehisa
- <i>Department of Biotechnology, Faculty of Engineering, Kansai University</i>
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- OBATA Hitoshi
- <i>Department of Biotechnology, Faculty of Engineering, Kansai University</i>
Bibliographic Information
- Other Title
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- Identification of a Novel Anti-ice-nucleating Polysaccharide from<i>Bacillus thuringiensis</i>YY529
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Abstract
Strain YY529, capable of producing some anti-ice-nucleating materials (ANM), was isolated from the surface of a camphor leaf. Strain YY529 was identified as Bacillus thuringiensis from its characteristics and taxonomy; the optimum temperature and pH for producing these ANMs were 30°C and 7.0, respectively. One of the ANM with the highest activities among them was purified from the culture. The molecular weight of the ANM was approximately 130 kDa based on a gel filtration analysis. We confirmed that this ANM was a polysaccharide based on the results of the treatment with a mannosidase and the molish reaction. In addition, the LCMS analysis showed that this anti-ice-nucleating polysaccharide (ANPS) had the polyacetyl-D-glucosamine moiety in its structure. Furthermore, this ANPS showed its ability as a non-freeze agent in a preservative solution for the cryopreservation of cock liver. This is the first report of ANPS as a novel ANM from Bacillus thuringiensis YY529.<br>
Journal
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 66 (5), 948-954, 2002
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Details 詳細情報について
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- CRID
- 1390282681449956992
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- NII Article ID
- 110002693763
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- NII Book ID
- AA10824164
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- COI
- 1:CAS:528:DC%2BD38XktlSkurc%3D
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- ISSN
- 13476947
- 09168451
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- NDL BIB ID
- 6179904
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- PubMed
- 12092845
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed