Molecular Cloning and Overexpression of fleA Gene Encoding a Fucose-specific Lectin of Aspergillus oryzae.

• ISHIDA Hiroki

  <i>Research Institute, Gekkeikan Sake Co. Ltd.</i>

• MORITANI Toshiyasu

  <i>Research Institute, Gekkeikan Sake Co. Ltd.</i>

• HATA Yoji

  <i>Research Institute, Gekkeikan Sake Co. Ltd.</i>

• KAWATO Akitsugu

  <i>Research Institute, Gekkeikan Sake Co. Ltd.</i>

• SUGINAMI Koji

  <i>Research Institute, Gekkeikan Sake Co. Ltd.</i>

• ABE Yasuhisa

  <i>Research Institute, Gekkeikan Sake Co. Ltd.</i>

• IMAYASU Satoshi

  <i>Research Institute, Gekkeikan Sake Co. Ltd.</i>

Bibliographic Information

Other Title

• Molecular Cloning and Overexpression of<i>fleA</i>Gene Encoding a Fucose-specific Lectin of<i>Aspergillus oryzae</i>

Abstract

  A protein from the cell lysate of Aspergillus oryzae was purified by column chromatography immobilized with a ferrichrysin (Fcy), which is one of the siderophores of A. oryzae. It is produced only in an iron-deficient culture and its molecular weight is estimated as 35,000 by SDS-PAGE. Two internal amino acid sequences of the protein obtained by lysylendopeptidase digestion were analyzed. Molecular cloning shows that it encodes 310 putative amino acid residues separated by 4 introns and is designated as fleA. It shows approximately 26% similarity with the gene encoding a fucose-specific lectin of Aleuria aurantia (AAL). The gene was overexpressed under control of the melO promoter in a submerged culture of A. oryzae. The fleA gene product showed hemagglutination activity against rabbit erythrocytes. A hemagglutination inhibition assay of monosaccharides showed that this lectin specifically binds to L-fucose and weakly reacts with mannose and N-acetyl-neuraminic acid.<br>

Journal

• Bioscience, Biotechnology, and Biochemistry

  Bioscience, Biotechnology, and Biochemistry 66 (5), 1002-1008, 2002

  Japan Society for Bioscience, Biotechnology, and Agrochemistry

Keywords

• fucose-specific lectin
• <i>Aspergillus oryzae</i>
• <i>fleA</i>
• <i>melO</i> promoter
• ferrichrysin

Details

• CRID: 1390001206473253248
• NII Article ID: 110002693770
• NII Book ID: AA10824164
• DOI: 10.1271/bbb.66.1002
• COI: 1:CAS:528:DC%2BD38XktlSku7g%3D
• ISSN: 13476947
• ISSN: 09168451
• NDL BIB ID: 6180104
• PubMed: 12092808
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6180104
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.66.1002
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• Abstract License Flag: Disallowed