Primary Structure and Catalytic Properties of a Cold-active Esterase from a Psychrotroph, <i>Acinetobacter</i> sp. Strain No. 6. Isolated from Siberian Soil
-
- SUZUKI Takeshi
- <i>Laboratory of Microbial Biochemistry, Institute for Chemical Research, Kyoto University</i>
-
- NAKAYAMA Toru
- <i>Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University</i>
-
- KURIHARA Tatsuo
- <i>Laboratory of Microbial Biochemistry, Institute for Chemical Research, Kyoto University</i>
-
- NISHINO Tokuzo
- <i>Department of Biomolecular Engineering, Graduate School of Engineering, Tohoku University</i>
-
- ESAKI Nobuyoshi
- <i>Laboratory of Microbial Biochemistry, Institute for Chemical Research, Kyoto University</i>
書誌事項
- タイトル別名
-
- Primary Structure and Catalytic Properties of a Cold-active Esterase from a Psychrotroph, Acinetobacter sp. Strain No. 6. Isolated from Siberian Soil.
この論文をさがす
抄録
We cloned a gene coding for a cold-active esterase from a genomic library of Acinetobacter sp. strain No. 6, a psychrotroph isolated from Siberian soil. The gene, aest, encoded a protein of 301 amino acid residues, the deduced sequence of which had less than 17% identity to sequences of known esterases and lipases. However, the esterase seemed to belong to the α/β hydrolase superfamily, because it contained a sequence, Gly-Xaa-Ser-Xaa-Gly (with Xaa an arbitrary amino acid residue), found in most serine hydrolases of this superfamily. Sequence comparison earlier suggested a weak phylogenetic relationship of gene product AEST to the EST group of the esterase-lipase family, which has been found only in eukaryotes. The aest gene was expressed in Escherichia coli BL21(DE3) cells under the control of the T7 promoter, and the expression product was purified to homogeneity and characterized. It catalyzed the hydrolysis of esters with short-chain acyl groups and had lower activation energy and lower thermostability than do mesophilic enzymes, as expected from the cold-adapted nature of this enzyme.<br>
収録刊行物
-
- Bioscience, Biotechnology, and Biochemistry
-
Bioscience, Biotechnology, and Biochemistry 66 (8), 1682-1690, 2002
公益社団法人 日本農芸化学会
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1390282681450240768
-
- NII論文ID
- 110002693885
-
- NII書誌ID
- AA10824164
-
- COI
- 1:STN:280:DC%2BD38vpt1aksw%3D%3D
-
- ISSN
- 13476947
- 09168451
-
- NDL書誌ID
- 6286943
-
- PubMed
- 12353628
-
- 本文言語コード
- en
-
- データソース種別
-
- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
-
- 抄録ライセンスフラグ
- 使用不可