Continuous Cell-free Protein Synthesis Directed by Messenger DNA and Catalyzed by Extract of<i>Thermus thermophilus</i>HB27

• UZAWA Taketoshi

  <i>Department of Life Science, Tokyo Institute of Technology</i> Present address: <i>Division of Natural Science, Osaka Kyoiku University</i>

• YAMAGISHI Akihiko

  <i>Department of Life Science, Tokyo Institute of Technology</i> Present address: <i>Faculty of Life Science, Tokyo University of Pharmacy and Life Science</i>

• OSHIMA Tairo

  <i>Department of Life Science, Tokyo Institute of Technology</i> Present address: <i>Faculty of Life Science, Tokyo University of Pharmacy and Life Science</i>

書誌事項

タイトル別名

• Continuous Cell-free Protein Synthesis Directed by Messenger DNA and Catalyzed by Extract of Thermus thermophilus HB27

抄録

  Polypeptide synthesis directed by DNA as the messenger in a cell-free system of Thermus thermophilus was investigated. Polypeptides were synthesized with the addition of neomycin in the presence of DNA catalyzed by the cell extract. The stability of messenger DNA was greater than that of messenger RNA. Continuous cell-free translation with messenger DNA produced polypeptides at the rate of more than 8 μg/h in the presence of spermine.<br>

収録刊行物

• Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ

  Ｂｉｏｓｃｉｅｎｃｅ，　Ｂｉｏｔｅｃｈｎｏｌｏｇｙ，　ａｎｄ　Ｂｉｏｃｈｅｍｉｓｔｒｙ 67 (3), 639-642, 2003

  公益社団法人 日本農芸化学会

キーワード

• <i>Thermus thermophilus</i>
• messenger DNA
• cell-free protein synthesis
• extreme thermophile
• polyamine

詳細情報

• CRID: 1390001206476473088
• NII論文ID: 110002694112
• NII書誌ID: AA10824164
• DOI: 10.1271/bbb.67.639
• COI: 1:CAS:528:DC%2BD3sXivFKqu7k%3D
• ISSN: 13476947
• ISSN: 09168451
• NDL書誌ID: 6494187
• PubMed: 12723617
• Web Site: http://id.ndl.go.jp/bib/6494187
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6494187
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.67.639
• 本文言語コード: en
• データソース種別:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可