Targeted Gene Disruption of the Neuronal Calcium Sensor 1 Homologue in Rice Blast Fungus,<i>Magnaporthe grisea</i>

  • SAITOH Ken-ichiro
    <i>Faculty of Agriculture, Tokyo University of Agriculture and Technology</i>
  • ARIE Tsutomu
    <i>Faculty of Agriculture, Tokyo University of Agriculture and Technology</i>
  • TERAOKA Tohru
    <i>Faculty of Agriculture, Tokyo University of Agriculture and Technology</i>
  • YAMAGUCHI Isamu
    <i>Microbial Toxicology Laboratory, RIKEN</i> Present address: <i>Chemical Genetics Laboratory, RIKEN</i>
  • KAMAKURA Takashi
    <i>Microbial Toxicology Laboratory, RIKEN</i>

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  • Targeted Gene Disruption of the Neuronal Calcium Sensor 1 Homologue in Rice Blast Fungus, Magnaporthe grisea

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  We isolated a neuronal calcium sensor 1/frequenin-like gene, Mg-NCS-1, from Magnaporthe grisea and evaluated the phenotypes of null-mutants of the gene. The putative Mg-NCS-1 protein showed high similarity to the other NCS-1 proteins. The null-mutants had normal growth and pathogenicity similar to the parental strain, but their growth was suppressed in high concentrations of Ca2+ or acidic conditions.<br>

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