Expression in Cereal Plants of Genes That Inactivate Fusarium Mycotoxins

• HIGA Arisa

  <i>Laboratory for Remediation Research, Plant Science Center, RIKEN</i>

• KIMURA Makoto

  <i>Laboratory for Remediation Research, Plant Science Center, RIKEN</i>

• MIMORI Kouhei

  <i>Laboratory for Remediation Research, Plant Science Center, RIKEN</i> <i>Department of Engineering, Toyo University</i>

• OCHIAI-FUKUDA Tetsuko

  <i>Laboratory for Remediation Research, Plant Science Center, RIKEN</i>

• TOKAI Takeshi

  <i>Laboratory for Remediation Research, Plant Science Center, RIKEN</i> <i>Department of Life Science, Toyo University</i>

• TAKAHASHI-ANDO Naoko

  <i>Laboratory for Remediation Research, Plant Science Center, RIKEN</i>

• NISHIUCHI Takumi

  <i>Laboratory for Remediation Research, Plant Science Center, RIKEN</i> <i>Institute for Gene Research, Kanazawa University</i>

• IGAWA Tomoko

  <i>Laboratory for Remediation Research, Plant Science Center, RIKEN</i>

• FUJIMURA Makoto

  <i>Department of Life Science, Toyo University</i>

• HAMAMOTO Hiroshi

  <i>Laboratory for Adaptation and Resistance, Plant Science Center, RIKEN</i>

• USAMI Ron

  <i>Department of Engineering, Toyo University</i>

• YAMAGUCHI Isamu

  <i>Laboratory for Remediation Research, Plant Science Center, RIKEN</i> <i>Laboratory for Adaptation and Resistance, Plant Science Center, RIKEN</i>

Bibliographic Information

Other Title

• Expression in Cereal Plants of Genes That Inactivate<i>Fusarium</i>Mycotoxins

Abstract

  Trichothecene 3-O-acetyltransferase (encoded by Tri101) inactivates the virulence factor of the cereal pathogen Fusarium graminearum. Zearalenone hydrolase (encoded by zhd101) detoxifies the oestrogenic mycotoxin produced by the same pathogen. These genes were introduced into a model monocotyledon rice plant to evaluate their usefulness for decontamination of mycotoxins. The strong and constitutive rice Act1 promoter did not cause accumulation of TRI101 protein in transgenic rice plants. In contrast, the same promoter was suitable for transgenic production of ZHD101 protein; so far, five promising T0 plants have been generated. Low transgenic expression of Tri101 was suggested to be increased by addition of an Ω enhancer sequence upstream of the start codon.<br>

Journal

• Bioscience, Biotechnology, and Biochemistry

  Bioscience, Biotechnology, and Biochemistry 67 (4), 914-918, 2003

  Japan Society for Bioscience, Biotechnology, and Agrochemistry

Keywords

• Fusarium head blight
• mycotoxin and phytotoxin
• transgenic cereals
• trichothecene resistance
• zearalenone detoxification

Details

• CRID: 1390001206475808256
• NII Article ID: 110002694157
• NII Book ID: AA10824164
• DOI: 10.1271/bbb.67.914
• COI: 1:CAS:528:DC%2BD3sXjslOmurc%3D
• ISSN: 13476947
• ISSN: 09168451
• NDL BIB ID: 6517413
• PubMed: 12784641
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6517413
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.67.914
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• Abstract License Flag: Disallowed