A T42M Substitution in Bacterial 5-Enolpyruvylshikimate-3-phosphate Synthase (EPSPS) Generates Enzymes with Increased Resistance to Glyphosate

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  • HE Ming
    <i>Biotechnology Research Center, The Key Laboratory of Gene Engineering of Education Ministry, Zhongshan University</i>
  • NIE Yan-Fang
    <i>Biotechnology Research Center, The Key Laboratory of Gene Engineering of Education Ministry, Zhongshan University</i>
  • XU Peilin
    <i>Biotechnology Research Center, The Key Laboratory of Gene Engineering of Education Ministry, Zhongshan University</i>

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  • T42M Substitution in Bacterial 5 Enolpyruvylshikimate 3 phosphate Synthase EPSPS Generates Enzymes with Increased Resistance to Glyphosate

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  Mutants of class I enolpyruvylshikimate 3-phosphate synthase (EPSPS) with resistance to glyphosate were produced in a previous study using the staggered extension process with aroA genes from S. typhimurium and E. coli. Two of these mutants shared a common amino acid substitution, T42M, near the hinge region between the large globular domains of EPSPS. Using site-directed mutagenisis, we produced the T42M mutants without the other amino acid changes of the original mutants. The T42M substitution alone produced enzymes with a 9- to 25-fold decreased Km[PEP] and a 21- to 26-fold increased Ki[glyphosate] compared to the wild-type enzymes. These results provide more testimony for the powerful approach for protein engineering by the combination of directed evolution and rational design.<br>

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