Stimulation of Glucose Uptake in Muscle Cells by Prolonged Treatment with Scriptide, a Histone Deacetylase Inhibitor
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- TAKIGAWA-IMAMURA Hisako
- <i>Medicinal Research Laboratories, Taisho Pharmaceutical Co., Ltd.</i>
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- SEKINE Takumi
- <i>Medicinal Research Laboratories, Taisho Pharmaceutical Co., Ltd.</i>
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- MURATA Mitsuo
- <i>Medicinal Research Laboratories, Taisho Pharmaceutical Co., Ltd.</i>
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- TAKAYAMA Kiyoshi
- <i>Medicinal Research Laboratories, Taisho Pharmaceutical Co., Ltd.</i>
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- NAKAZAWA Kiyoshi
- <i>Medicinal Research Laboratories, Taisho Pharmaceutical Co., Ltd.</i>
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- NAKAGAWA Junichi
- <i>Medicinal Research Laboratories, Taisho Pharmaceutical Co., Ltd.</i>
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Abstract
Glucose incorporation is regulated mainly by GLUT4 in skeletal muscles. Here we report that treatment of L6 myotubes with scriptide, a hydroxamic acid-based histone deacetylase (HDAC) inhibitor, stimulated 2-deoxyglucose uptake. The effect appeared only after 24 hr, resulting in 2.4-fold glucose uptake at treatment day 6. Scriptide acted synergistically with insulin, indicating it stimulated a distinct pathway from the insulin signaling pathway. It was not observed in undifferentiated myoblasts or 3T3-L1 adipocytes, suggesting a muscle-specific effect of scriptide. A five-carbon chain and hydroxamic acid, essential for histone deacetylase inhibition, were indispensable for this effect, and trichostatin A stimulated glucose uptake as well. Scriptide increased the cellular content of GLUT4, and induced GLUT4 translocation, but GLUT4 mRNA level did not change, indicating scriptide functions posttranslationally. Our results indicated a novel function for HDAC inhibitors of increasing GLUT4 content and its translocation in muscle cells, resulting in stimulation of glucose uptake.<br>
Journal
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- Bioscience, Biotechnology, and Biochemistry
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Bioscience, Biotechnology, and Biochemistry 67 (7), 1499-1506, 2003
Japan Society for Bioscience, Biotechnology, and Agrochemistry
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Details 詳細情報について
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- CRID
- 1390001206473566208
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- NII Article ID
- 110002694255
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- NII Book ID
- AA10824164
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- COI
- 1:CAS:528:DC%2BD3sXmtFelu70%3D
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- ISSN
- 13476947
- 09168451
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- NDL BIB ID
- 6622006
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- PubMed
- 12913293
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- Text Lang
- en
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- Data Source
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- JaLC
- NDL
- Crossref
- PubMed
- CiNii Articles
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- Abstract License Flag
- Disallowed