Conversion of the Cleavage Specificity of Subtilisin YaB on Oxidized Insulin Chains to an Elastase-like Specificity by Replacement of Gly124 with Ala

• MEI Hui-Ching

  <i>Institute of Biochemistry, National Yang-Ming University</i> <i>Department of Medical Technology, China Medical College</i>

• LI Ywan-Feng

  <i>Institute of Biochemistry, National Yang-Ming University</i>

• HSU Chi-Cheng

  <i>Institute of Biochemistry, National Yang-Ming University</i>

• TSAI Ying-Chieh

  <i>Institute of Biochemistry, National Yang-Ming University</i>

• TAKAGI Hiroshi

  <i>Department of Bioscience, Fukui Prefectural University</i>

Abstract

  Replacement of Gly124 on the S1 pocket of subtilisin YaB with Ala changed the cleavage pattern on oxidized insulin B-chain from the subtilisin type to the elastase type. The initial cleavage site in the B-chain shifted from L15-Y16 for wild-type YaB to A14-L15 for the G124A mutant. Upon complete hydrolysis with the G124A mutant, four of the six major cleavage sites on the B-chain were identical to porcine pancreatic elastase cleavage sites.<br>

Journal

• Bioscience, Biotechnology, and Biochemistry

  Bioscience, Biotechnology, and Biochemistry 67 (7), 1601-1604, 2003

  Japan Society for Bioscience, Biotechnology, and Agrochemistry

Keywords

• subtilisin YaB
• substrate specificity
• oxidized insulin chains

Details

• CRID: 1390001206473763200
• NII Article ID: 110002694273
• NII Book ID: AA10824164
• DOI: 10.1271/bbb.67.1601
• COI: 1:CAS:528:DC%2BD3sXmtFeks78%3D
• ISSN: 13476947
• ISSN: 09168451
• NDL BIB ID: 6622282
• PubMed: 12913311
• Web Site: https://ndlsearch.ndl.go.jp/books/R000000004-I6622282
• Web Site: http://www.tandfonline.com/doi/pdf/10.1271/bbb.67.1601
• Web Site: https://search.jamas.or.jp/link/ui/2004206191
• Text Lang: en
• Data Source:
  • JaLC
  • NDL
  • Crossref
  • PubMed
  • CiNii Articles
• Abstract License Flag: Disallowed