Properties of a Novel Extracellular Cell-free Ice Nuclei from Ice-nucleating Pseudomonas antarctica IN-74

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  • MURYOI Naomi
    <i>Department of Biotechnology, Faculty of Engineering, Kansai University</i>
  • KAWAHARA Hidehisa
    <i>Department of Biotechnology, Faculty of Engineering, Kansai University</i>
  • OBATA Hitoshi
    <i>Department of Biotechnology, Faculty of Engineering, Kansai University</i>

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  • Properties of a Novel Extracellular Cell-free Ice Nuclei from Ice-nucleating <i>Pseudomonas antarctica</i> IN-74

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  Some ice-nucleating bacterial strains, including Pantoea ananatis (Erwinia uredovora), Pseudomonas fluorescens, and Pseudomonas syringae isolates, were examined for the ability to shed ice nuclei into the growth medium. A novel ice-nucleating bacterium, Pseudomonas antarctica IN-74, was isolated from Ross Island, Antarctica. Cell-free ice nuclei from P. antarctica IN-74 were different from the conventional cell-free ice nuclei and showed a unique characterization. Cell-free ice nuclei were purified by centrifugation, filtration (0.45 μm), ultrafiltration, and gel filtration. In an ice-nucleating medium in 1 liter of cell culture, maximum growth was obtained with the production of 1.9 mg of cell-free ice nuclei. Ice nucleation activity in these cell-free ice nuclei preparations was extremely sensitive to pH. It was demonstrated that the components of cell-free ice nuclei were protein (33%), saccharide (12%), and lipid (55%), indicating that cell-free ice nuclei were lipoglycoproteins. Also, carbohydrate and lipid stains showed that cell-free ice nuclei contained both carbohydrate and lipid moieties.<br>

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