Tributyltin (TBT) Increases TNF α mRNA Expression and Induces Apoptosis in the Murine Macrophage Cell Line in Vitro

• NAKANO Ken

  Fukushima Prefecture Ken-poku Public Health and Welfare Office Department of Public Health, Fukushima Medical University Fukushima Institute for Public Health

• TSUNODA Masashi

  Department of Public Health, Fukushima Medical University Department of Preventive Medicine and Public Health, Kitasato University School of Medicine

• KONNO Nobuhiro

  Department of Home Economics, Koriyama Women’s University and College

書誌事項

タイトル別名

• Tributyltin (TBT) Increases TNF .ALPHA. mRNA Expression and Induces Apoptosis in the Murine Macrophage Cell Line in Vitro

抄録

Objective: Tributyltin (TBT) compounds have been widely used as antifouling agents for ship-bottom paint. The immune system is a target of TBT intoxication. We evaluated the effects of TBT chloride in macrophages, which have critical roles in the immune system, using a murine macrophage lineage cell line, J774.1, in vitro.<br> Methods: We examined tumor necrosis factor α (TNF α), interleukin-1β (IL-1β) and c-jun mRNA expression in J774.1 cells. The effects of TBT on the apoptosis of J774.1 cells were examined by determining the percentage of TUNEL-positive cells and caspase-3 activity.<br> Results: The mean values of the viabilities of J774.1 cells exposed to TBT decreased dose-dependently. The relative mRNA expression of TNFα increased dose-dependently, however, that of IL-1β was not significantly different among the groups. The mean percentage of TUNEL-positive cells increased dose-dependently. Increases in the caspase-3 activities of J774.1 cells were observed in the groups exposed to higher concentrations of TBT. The mean value of relative mRNA expression of c-Jun transcription factor increased dose-dependently.<br> Conclusions: The increases in the percentage of TUNEL-positive cells and in caspase-3 activity suggested that exposure to TBT induces apoptosis of J774.1 cells. The increases in the mRNA expression of TNFα and c-jun by TBT may be related to apoptosis in macrophages.<br>

収録刊行物

• Environmental Health and Preventive Medicine

  Environmental Health and Preventive Medicine 9 (6), 266-271, 2004

  一般社団法人日本衛生学会

キーワード

• tributyltin
• immunotoxicity
• macrophage
• apoptosis

詳細情報

• CRID: 1390001204459214080
• NII論文ID: 110002694784
• NII書誌ID: AA1108348X
• DOI: 10.1265/ehpm.9.266
• ISSN: 13474715
• ISSN: 1342078X
• Web Site: https://search.jamas.or.jp/link/ui/2006137232
• 本文言語コード: en
• データソース種別:
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  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可